Chadelat K, Boule M, Corroyer S, Fauroux B, Delaisi B, Tournier G, Clement A
Dept of Pediatric Pulmonology, Trousseau Hospital, St Antoine Medical School, University of Paris, France.
Eur Respir J. 1998 Jun;11(6):1329-36. doi: 10.1183/09031936.98.11061329.
The involvement of the insulin-like growth factor (IGF) system in lung growth and repair following injury is sustained by a number of studies. Based on this knowledge, the aim of the present work was to document the expression of the IGFs and their binding proteins by alveolar cells obtained by bronchoalveolar lavage (BAL). Two groups were investigated: a control group of five children and a group of 11 children referred to the department for exploration of interstitial lung disease (ILD). Components of the IGF system studied included IGF-I, IGF-II and IGF-binding proteins (IGFBP). Expression of these factors was analysed at the level of messenger ribonucleic acid (mRNA) (by semi-quantitative reverse transcription polymerase chain reaction techniques), and of protein for the IGFBPs. In addition, expression of two major cytokines associated with the inflammatory process, tumour necrosis factor-alpha (TNF-alpha) and transforming growth factor-beta (TGF-beta), was also documented. In children without parenchymal disease, the growth factor expressed was IGF-I, in association with the presence of mRNA for IGFBP-2 in all cases. In children with ILD, expression of IGF-I was observed in nine patients and of IGF-II in three patients, and the presence of IGFBP-2 was found in all extracts analysed (mRNA and proteins). Evaluation of IGFBP-2 expression indicated an increase in the group of children with ILD. Interestingly, a significant association was observed between the increase in IGFBP-2 expression and TGF-beta expression. The present data emphasize the presence on insulin-like growth factor-binding protein-2 in the BAL of all patients, and suggest that this protein may be an important factor of the injury/repair processes during the progression of alveolar inflammation.
多项研究证实胰岛素样生长因子（IGF）系统参与了肺损伤后的生长和修复过程。基于这一认识，本研究旨在通过支气管肺泡灌洗（BAL）获取的肺泡细胞来记录IGF及其结合蛋白的表达情况。研究对象分为两组：一组为5名儿童的对照组，另一组为11名因间质性肺疾病（ILD）前来该科室检查的儿童。所研究的IGF系统组成成分包括IGF-I、IGF-II和IGF结合蛋白（IGFBP）。通过信使核糖核酸（mRNA）水平（采用半定量逆转录聚合酶链反应技术）以及IGFBP的蛋白质水平对这些因子的表达进行分析。此外，还记录了与炎症过程相关的两种主要细胞因子——肿瘤坏死因子-α（TNF-α）和转化生长因子-β（TGF-β）的表达情况。在无实质性疾病的儿童中，所表达的生长因子为IGF-I，且所有病例中均存在IGFBP-2的mRNA。在患有ILD的儿童中，9例患者检测到IGF-I的表达，3例患者检测到IGF-II的表达，且在所有分析提取物（mRNA和蛋白质）中均发现了IGFBP-2。对IGFBP-2表达的评估表明，ILD患儿组该蛋白表达增加。有趣的是，观察到IGFBP-2表达增加与TGF-β表达之间存在显著关联。目前的数据强调了所有患者BAL中均存在胰岛素样生长因子结合蛋白-2，并表明该蛋白可能是肺泡炎症进展过程中损伤/修复过程的一个重要因素。
