Tessier V, Chadelat K, Baculard A, Housset B, Clement A
Department of Physiology, Hopital Trousseau, St. Antoine Medical School, University of Paris, France.
Chest. 1996 Jun;109(6):1430-8. doi: 10.1378/chest.109.6.1430.
The development of BAL in children for both research and clinical purposes has been limited so far by the difficulty in establishing reference values. The aim of the study was (1) to define composition of BAL cellular components in control children and to evaluate the ability of these cells to express various cytokines, and (2) to study modifications of differential cytology and BAL cell cytokine responses in children with interstitial lung disorders.
Two groups were investigated: a control group of 16 children who were concluded to be free of parenchymal lung disease after complete pulmonary investigation, and a group of 11 children with pulmonary sarcoidosis. Differential cytology was evaluated by standard techniques. BAL cell cytokine expression was studied at the level of messenger RNA (mRNA) by reverse transcription-polymerase chain reaction (RT-PCR) methods.
In the control group, differential cell counts appeared to be similar to values reported in adult populations with normal distribution of the data and no influence of age. In this group, no transcripts for interleukin-1beta (IL-1beta), tumor necrosis factor-alpha (TNF-alpha), IL-6, and transforming (correction of tranforming) growth factor-beta (TGF-beta) could be detected. In children with sarcoidosis, different profiles of IL-1beta, TNF-alpha, IL-6, and TGF-beta expression were individualized which seemed to be related to the activity and/or severity of the disease, IL-6 and TGF-beta mRNA being observed only in the more severe forms.
These data provide information on BAL cell number and function in children. Characterization of BAL cytokine expression patterns during the course of interstitial lung diseases in children may be of great interest for evaluation of disease activity and/or severity and therefore for planning of therapy.
到目前为止，由于难以建立参考值，用于儿童研究和临床目的的支气管肺泡灌洗（BAL）技术发展受限。本研究的目的是：（1）确定对照儿童BAL细胞成分的组成，并评估这些细胞表达各种细胞因子的能力；（2）研究间质性肺疾病患儿的细胞分类学变化及BAL细胞因子反应。
调查了两组儿童：一组为16名对照儿童，经全面肺部检查后确定无实质性肺部疾病；另一组为11名患有肺结节病的儿童。采用标准技术评估细胞分类学。通过逆转录-聚合酶链反应（RT-PCR）方法在信使核糖核酸（mRNA）水平研究BAL细胞因子表达。
在对照组中，细胞分类计数似乎与已报道的成年人群数值相似，数据呈正态分布，且不受年龄影响。该组未检测到白细胞介素-1β（IL-1β）、肿瘤坏死因子-α（TNF-α）、IL-6和转化生长因子-β（TGF-β）的转录本。在患有结节病的儿童中，IL-1β、TNF-α、IL-6和TGF-β的不同表达谱具有个体差异，这似乎与疾病的活动度和/或严重程度有关，仅在病情较严重的患儿中观察到IL-6和TGF-β mRNA。
这些数据提供了有关儿童BAL细胞数量和功能的信息。儿童间质性肺疾病过程中BAL细胞因子表达模式的特征化，对于评估疾病活动度和/或严重程度以及因此制定治疗方案可能具有重要意义。
