Krizanová H, Koppel Z, Horváth J, Beseda M, Halasa M
Vet Med (Praha). 1978 May;23(5):305-15.
The authors have been studying the bacterial diseases of the brood for a fairly long time. American and European foul brood has been studied with the highest attention. Cultures of Bacillus larvae (White, 1906) were examined both in freshly isolated strains and in collection strains of this micro-organism. In cases of foul brood, the pathological material was found to contain not only the typical rods of B. larvae but also immobile spiral forms which are usually referred to in literature as fragments or developmental forms of B. larvae. These spiral forms were found to constitute spindle-shaped formations in the culture of B. larvae; the multiplication of these spindles depends on the presence of the rods of B. larvae and their development and reproduction can be observed on wet gelatine agar in a Petri dish turned upside down under a normal microscope (10 X 10 magnification). In the combined liquid medium, used in the experiments, these formations disintegrate into immobile spirals; if re-cultivated on a solid medium they re-assume their spindle shape with transverse meridian arrangement (in different amounts). Staining for proving the presence of nucleic acids does not eliminate the possibility of these formations being separate micro-organisms which cannot be stained by current staining methods but can be represented by the contrast method according to Burri, or by silvering according to Klein. The authors succeeded to separate these micro-organisms, but without the rods of B. larvae the colonies of these formations are feeble.
作者们对幼虫期细菌性疾病进行了相当长时间的研究。对美洲幼虫腐臭病和欧洲幼虫腐臭病给予了高度关注。对幼虫芽孢杆菌（怀特，1906年）的培养物进行了检查，包括新分离的菌株和该微生物的保藏菌株。在幼虫腐臭病病例中，病理材料不仅发现含有典型的幼虫芽孢杆菌杆菌，还发现有不活动的螺旋形态，文献中通常将其称为幼虫芽孢杆菌的片段或发育形态。这些螺旋形态在幼虫芽孢杆菌培养物中形成纺锤形结构；这些纺锤体的增殖取决于幼虫芽孢杆菌杆菌的存在，并且在倒置的培养皿中的湿明胶琼脂上，在普通显微镜（10×10放大倍数）下可以观察到它们的发育和繁殖。在实验中使用的混合液体培养基中，这些结构分解为不活动的螺旋体；如果在固体培养基上重新培养，它们会重新呈现出带有横向子午线排列（数量不同）的纺锤形状。用于证明核酸存在的染色并不能排除这些结构是单独的微生物的可能性，这些微生物不能被当前的染色方法染色，但可以根据布里的对比法或根据克莱因的镀银法来显示。作者成功地分离出了这些微生物，但没有幼虫芽孢杆菌杆菌时，这些结构的菌落很微弱。
