Drobníková V
Vet Med (Praha). 1978 Mar;23(3):185-90.
The authoress studied the growth of Bacillus larvae on different nutrient media and its ability of decomposing hydrogen peroxide and reducing nitrates. There are also instructions for rapid cultivation and biochemical diagnosis of Bacillus larvae. It can be performed in any microbiological laboratory, the culture medium can be prepared from available commercially produced preparations, and B. larvae can be detected in suspected material even out of the season, if the method of selective cultivation and repeated pasteurizing is used. Catalase test is proposed to be added to the examination; the result of this test was negative in all 96 strains studied. Photographic documentation of different developmental stages of B. larvae can serve as an aid in microbiological examination.
该女作者研究了幼虫芽孢杆菌在不同营养培养基上的生长情况及其分解过氧化氢和还原硝酸盐的能力。文中还给出了幼虫芽孢杆菌快速培养和生化诊断的说明。该操作可在任何微生物实验室进行,培养基可由市售制剂配制而成,并且如果采用选择性培养和反复巴氏灭菌的方法,即使在非季节期间,也能在疑似材料中检测到幼虫芽孢杆菌。建议在检查中增加过氧化氢酶试验;在所研究的全部96株菌株中,该试验结果均为阴性。幼虫芽孢杆菌不同发育阶段的照片记录有助于微生物学检查。