Batzer R, Liu Y, Cochran D L, Szmuckler-Moncler S, Dean D D, Boyan B D, Schwartz Z
Department of Periodontics, University of Texas Health Center at San Antonio 78284, USA.
J Biomed Mater Res. 1998 Sep 5;41(3):489-96. doi: 10.1002/(sici)1097-4636(19980905)41:3<489::aid-jbm20>3.0.co;2-c.
Surface roughness affects proliferation, differentiation (alkaline phosphatase and osteocalcin), local factor production (transforming growth factor (TGF beta) and prostaglandin E2 (PGE2)], and response to 1,25-(OH)2D3 (1,25) of MG63 osteoblast-like cells. In this study, we examined whether the effect of surface roughness on MG63 cells is mediated by prostaglandins produced by the cells. Unalloyed titanium (Ti) disks were pretreated with HF/HNO3 (PT) and then machined and acid-etched (MA). Disks were also coarse grit-sandblasted (SB), coarse grit-sandblasted and acid-etched (CA), or plasma-sprayed with Ti particles (PS). The surfaces, from smoothest to roughest, were PT, MA, CA, SB, and PS. MG63 cells were cultured to confluence on the Ti disks in the presence or absence of 10(-7) M indomethacin (Indo), a specific inhibitor of cyclooxygenase activity, resulting in decreased prostaglandin production. When the cells reached confluence, cell number, cell layer alkaline phosphatase specific activity (ALPase), and osteocalcin (OC) and latent TGF beta (LTGF beta) production were determined. In addition, confluent cultures which had been grown in the absence of Indo were exposed to 10(-7) M 1,25, 10(-7) M Indo, or a combination of the two for 24 h. On the rougher surfaces, cell number was decreased and ALPase, OC, and LTGF beta were increased. When indomethacin was present throughout the culture period, the effect of surface roughness on cell number, OC, and LTGF beta was abolished. ALPase was reduced, but surface roughness-dependent effects were still observed. Addition of indomethacin to confluent cultures for 24 h had no effect on any of the parameters examined, with one exception: Cells cultured on MA surfaces exhibited a more differentiated phenotype. 1,25 increased all parameters examined on SB, CA, and PS surfaces. When indomethacin was added with 1,25, the 1,25-dependent effects on cell number and OC and LTGF beta production were abolished; however, ALPase was unaffected. This indicates that bone cell response to systemic hormones may be modified by implant surface roughness. This effect may be mediated, at least in part, by prostaglandins produced by the same cells.
表面粗糙度会影响MG63成骨样细胞的增殖、分化（碱性磷酸酶和骨钙素）、局部因子产生（转化生长因子（TGF-β）和前列腺素E2（PGE2））以及对1,25-(OH)2D3（1,25）的反应。在本研究中，我们检测了表面粗糙度对MG63细胞的影响是否由细胞产生的前列腺素介导。将非合金钛（Ti）盘用HF/HNO3预处理（PT），然后进行机械加工和酸蚀（MA）。盘还进行了粗粒度喷砂处理（SB）、粗粒度喷砂和酸蚀处理（CA）或用Ti颗粒进行等离子喷涂（PS）。从最光滑到最粗糙的表面依次为PT、MA、CA、SB和PS。在存在或不存在10^(-7) M消炎痛（Indo）（一种环氧化酶活性的特异性抑制剂，可导致前列腺素产生减少）的情况下，将MG63细胞在Ti盘上培养至汇合。当细胞达到汇合时，测定细胞数量、细胞层碱性磷酸酶比活性（ALPase）、骨钙素（OC）和潜伏TGF-β（LTGF-β）的产生。此外，将在不存在Indo的情况下生长的汇合培养物暴露于10^(-7) M 1,25、10^(-7) M Indo或两者的组合中24小时。在较粗糙的表面上，细胞数量减少，而ALPase、OC和LTGF-β增加。当在整个培养期间存在消炎痛时，表面粗糙度对细胞数量、OC和LTGF-β的影响被消除。ALPase降低，但仍观察到表面粗糙度依赖性效应。将消炎痛添加到汇合培养物中24小时对所检测的任何参数均无影响，但有一个例外：在MA表面培养的细胞表现出更分化的表型。1,25增加了在SB、CA和PS表面上检测的所有参数。当消炎痛与1,25一起添加时，1,25对细胞数量以及OC和LTGF-β产生的依赖性效应被消除；然而，ALPase不受影响。这表明骨细胞对全身激素的反应可能会因植入物表面粗糙度而改变。这种效应可能至少部分地由同一细胞产生的前列腺素介导。
