Wilson D L, Szivek J A, Anderson P L, Miera V L, Battraw G A
Department of Surgery, Arizona Health Sciences Center, Tucson, USA.
J Invest Surg. 1998 Jan-Feb;11(1):29-48. doi: 10.3109/08941939809032178.
Identification of the strains controlling bone remodeling is important for determining ways to prevent bone loss due to load deprivation, or implant placement. Long-term monitoring of strains can potentially provide the best information. Glues are resorbed within 2-3 weeks. Two formulations of microcrystalline hydroxyapatite (HA) were used to attach strain gages to rat femora to assess their long-term in vivo strain measurement capability. Seven male rats received HA-coated gages, and 2 animals underwent a sham procedure. The gages were prepared using a published technique and placed on the antero-lateral aspect of the left femora. After 6-7 weeks, the animals were euthanized and both femora explanted. Gages were attached to the right femora with cyanoacrylate. All femora were tested in cantilever bending, then embedded, sectioned, and stained with mineralized bone stain. The undecalcified sections were examined using transmitted and ultraviolet light microscopy. Mechanical testing showed one HA formulation provided 70-100% bonding. Histology showed intimate contact between the gage and bone surface. Histomorphometry indicated increased bone activity under the gage compared to the remaining bone, the controls, and the shams. The results indicate that microcrystalline HAs bond to bone quickly and can allow long term in vivo measurements.
识别控制骨重塑的菌株对于确定预防因负荷剥夺或植入物放置导致骨质流失的方法很重要。对菌株进行长期监测可能会提供最佳信息。胶水在2-3周内会被吸收。使用两种微晶羟基磷灰石（HA）配方将应变片附着到大鼠股骨上，以评估它们在体内进行长期应变测量的能力。七只雄性大鼠接受了HA涂层应变片，两只动物进行了假手术。应变片采用已发表的技术制备，并放置在左股骨的前外侧。6-7周后，对动物实施安乐死并取出双侧股骨。用氰基丙烯酸酯将应变片附着到右侧股骨上。所有股骨都进行悬臂弯曲测试，然后进行包埋、切片，并用矿化骨染色剂染色。使用透射光和紫外光显微镜检查未脱钙切片。力学测试表明一种HA配方的粘结率为70-100%。组织学显示应变片与骨表面紧密接触。组织形态计量学表明，与其余骨骼、对照组和假手术组相比，应变片下方的骨活性增加。结果表明，微晶HA能迅速与骨结合，并可实现长期体内测量。
