Kawakami S, Callicott R H, Zhang N
Procter and Gamble Asia, Kobe Technical Center, Japan.
Analyst. 1998 Mar;123(3):489-91. doi: 10.1039/a706404g.
A method for the analysis of trace-level benzalkonium chloride has been established using flow injection ionspray mass spectrometry-mass spectrometry with multiple reaction monitoring. Quantification was carried out using an external standard based on peak area summation of each benzalkonium ion (C8, C10, C12, C14, C16 and C18) in the mass spectra. The multiple reaction monitoring technique provides additional specificity for identification and quantification. The quantification linear dynamic range was found to be 5.0-100.0 ng ml-1, the correlation coefficient > 0.999, and the detection limit 1.2 ng ml-1. The method was applied to quantify benzalkonium chloride on skin, which was sampled with a D-SQUAME tape from skin surface and extracted from the tape with methanol.
建立了一种使用流动注射离子喷雾质谱-质谱联用多反应监测技术分析痕量苯扎氯铵的方法。基于质谱图中各苯扎氯铵离子（C8、C10、C12、C14、C16和C18）的峰面积总和，采用外标法进行定量。多反应监测技术为鉴定和定量提供了额外的特异性。定量线性动态范围为5.0 - 100.0 ng ml-1，相关系数>0.999，检测限为1.2 ng ml-1。该方法应用于定量皮肤表面的苯扎氯铵，使用D-SQUAME胶带从皮肤表面取样，并用甲醇从胶带上提取。
