Lancaster F E
Department of Biology, Texas Woman's University, Denton 76204-3799, USA.
Alcohol Clin Exp Res. 1998 Jun;22(4):830-6.
Models for early stress and voluntary drinking were used to determine the contribution of early stress to increased intake of alcoholic beverages during puberty and adulthood. Newborn litters of Long Evans rats were: (1) stressed by daily separation from the mother for 15 min/day on days 1 to 7 of life ["handled" (H)]; or (2) left untouched with the mother on days 1 to 7 of life ["nonhandled" (NH)]. All animals were weaned on day 22, separated by sex (M and F), and caged individually with an assignment of 10 animals per sex per treatment group (H and NH). From 25 to 85 days of age, all animals were given free access to beer containing 5% ethanol (v/v), water, and regular laboratory food. Beer, food, and water intake was measured daily at the same time each day, and animals were weighed weekly. HM had greater ethanol intake and preference for ethanol during the peripubertal period (days 32 to 45), compared with all other groups. There were no differences in ethanol intake between NHF and NHM. HM had greater ethanol preference than HF on 22 of the 60 drinking days. HF consumed the same amount of water as the males and significantly greater amounts of water than NHF on 28 of the 60 drinking days. HM had greater ethanol preference than NHM on 8 of the 60 drinking days. From day 75 to day 85, HF had greater ethanol intake than HM, and NHF had greater ethanol intake than NHM. There were no differences in body weights of HF and NHF throughout the study. Growth of HM lagged behind NHM into adulthood. Early stress of males was linked to increased ethanol intake during the peripubertal and adult periods and stunted body growth into adulthood. Early stress of females was linked to polydipsia (water) throughout development and continuing into adulthood, and to increased alcohol intake in adulthood.
采用早期应激和自愿饮酒模型来确定早期应激对青春期和成年期酒精饮料摄入量增加的影响。将新生的Long Evans大鼠幼崽分为：(1) 在出生后第1至7天每天与母亲分离15分钟进行应激处理 ["处理组" (H)]；或(2) 在出生后第1至7天不做处理，与母亲待在一起 ["未处理组" (NH)]。所有动物在第22天断奶，按性别分开（雄性和雌性），每个处理组（H和NH）按性别将10只动物单独关笼饲养。从25日龄到85日龄，所有动物均可自由获取含5%乙醇（体积/体积）的啤酒、水和常规实验室食物。每天在同一时间测量啤酒、食物和水的摄入量，每周称动物体重。与所有其他组相比，处理组雄性在青春期前后（第32至45天）乙醇摄入量更高，对乙醇的偏好更强。未处理组雌性和未处理组雄性之间的乙醇摄入量没有差异。在60个饮酒日中的22天，处理组雄性对乙醇的偏好高于处理组雌性。在60个饮酒日中的28天，处理组雌性摄入的水量与雄性相同，且显著高于未处理组雌性。在60个饮酒日中的8天，处理组雄性对乙醇的偏好高于未处理组雄性。从第75天到第85天，处理组雌性的乙醇摄入量高于处理组雄性，未处理组雌性的乙醇摄入量高于未处理组雄性。在整个研究过程中，处理组雌性和未处理组雌性的体重没有差异。处理组雄性的生长在成年期滞后于未处理组雄性。雄性的早期应激与青春期前后和成年期乙醇摄入量增加以及成年期身体生长发育迟缓有关。雌性的早期应激与整个发育过程直至成年期的多饮（水）以及成年期酒精摄入量增加有关。
