XRCC2和XRCC3是新发现的人类Rad51家族成员，它们可促进染色体稳定性，并抵御DNA交联及其他损伤。

XRCC2 and XRCC3, new human Rad51-family members, promote chromosome stability and protect against DNA cross-links and other damages.

作者信息

Liu N, Lamerdin J E, Tebbs R S, Schild D, Tucker J D, Shen M R, Brookman K W, Siciliano M J, Walter C A, Fan W, Narayana L S, Zhou Z Q, Adamson A W, Sorensen K J, Chen D J, Jones N J, Thompson L H

机构信息

Biology and Biotechnology Research Program, Lawrence Livermore National Laboratory, Livermore, California 94551, USA.

出版信息

Mol Cell. 1998 May;1(6):783-93. doi: 10.1016/s1097-2765(00)80078-7.

DOI:10.1016/s1097-2765(00)80078-7

PMID:9660962

Abstract

The phenotypically similar hamster mutants irs1 and irs1SF exhibit high spontaneous chromosome instability and broad-spectrum mutagen sensitivity, including extreme sensitivity to DNA cross-linking agents. The human XRCC2 and XRCC3 genes, which functionally complement irs1 and irs1SF, respectively, were previously mapped in somatic cell hybrids. Characterization of these genes and sequence alignments reveal that XRCC2 and XRCC3 are members of an emerging family of Rad51-related proteins that likely participate in homologous recombination to maintain chromosome stability and repair DNA damage. XRCC3 is shown to interact directly with HsRad51, and like Rad55 and Rad57 in yeast, may cooperate with HsRad51 during recombinational repair. Analysis of the XRCC2 mutation in irs1 implies that XRCC2's function is not essential for viability in cultured hamster cells.

摘要

表型相似的仓鼠突变体irs1和irs1SF表现出高自发染色体不稳定性和广谱诱变敏感性，包括对DNA交联剂的极端敏感性。先前在体细胞杂种中定位了分别在功能上互补irs1和irs1SF的人类XRCC2和XRCC3基因。对这些基因的表征和序列比对表明，XRCC2和XRCC3是一个新出现的与Rad51相关的蛋白质家族的成员，它们可能参与同源重组以维持染色体稳定性和修复DNA损伤。XRCC3被证明可直接与HsRad51相互作用，并且与酵母中的Rad55和Rad57一样，可能在重组修复过程中与HsRad51协同作用。对irs1中XRCC2突变的分析表明，XRCC2的功能对于培养的仓鼠细胞的生存力并非必不可少。