[绿脓杆菌细胞成分的免疫学研究。III. 水溶性抗原复合物的免疫化学分析、毒性及保护特性]

[Immunologic study of the cellular components of bacillus pyocyaneus. III. Immunochemical analysis, toxicity and protective properties of water-soluble antigenic complexes].

作者信息

Stanislavskiĭ E S, Kolker I I, Grishina I A, Zhvanetskaia M I

出版信息

Zh Mikrobiol Epidemiol Immunobiol. 1978 Mar(3):65-70.

Abstract

Aqueous extracts of two Ps. aeruginosa strains killed with acetone were subjected to fractionation by preparative ultracentrifugation and gel-chromatography. Toxic activity of the extract was found to be connected with the high-molecular, possibly glycoprotein components reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 30--40% of rats against the generalized infection with Pseudomonas aeruginosa. This protective activity is apparently connected with the protein components (molecular weight--20000--60000 dalton), nontoxic for mice, not reacting with the corresponding antiserum in the immunoprecipitation test, and protecting 60 to 80% of rats against Ps. aeruginosa infecsion. Thus, as a result of preparative ultracentrifugation and gel-chromatography it was postible to divide the toxic and the nontoxic protective components of Ps. aeruginosa.

摘要

用丙酮灭活的两种铜绿假单胞菌菌株的水提取物，通过制备性超速离心和凝胶色谱法进行分级分离。提取物的毒性活性与高分子量、可能是糖蛋白的成分有关，这些成分在免疫沉淀试验中与相应抗血清发生反应，并能保护30% - 40%的大鼠免受铜绿假单胞菌的全身感染。这种保护活性显然与蛋白质成分（分子量为20000 - 60000道尔顿）有关，这些蛋白质成分对小鼠无毒，在免疫沉淀试验中不与相应抗血清发生反应，并能保护60%至80%的大鼠免受铜绿假单胞菌感染。因此，通过制备性超速离心和凝胶色谱法，有可能分离出铜绿假单胞菌的有毒和无毒保护成分。