Lee H, Williams S K, Giddings J C
Department of Chemistry, University of Utah, Salt Lake City 84112, USA.
Anal Chem. 1998 Jul 1;70(13):2495-503. doi: 10.1021/ac9710792.
An on-line concentration method has been developed for the analysis of dilute suspensions of macromolecules and particles by flow field-flow fractionation (FFF). This method, termed opposed flow sample concentration (OFSC), uses two opposing flowstreams to focus sample into a narrow band near the inlet of the flow FFF channel. Large sample volumes (up to 1 L) have been successfully loaded, concentrated, and separated in a 1 mL volume flow FFF channel. Concentration factors as high as 10(5) have been achieved. The effectiveness of OFSC flow FFF was demonstrated using mixtures of monodispersed standards such as proteins and polystyrene latex beads. Retention times, peak areas, and resolution are comparable for a 10 mL and a typical 10 microL injection volume. The applicability of the method to environmental studies was demonstrated by a separation of river water colloids.
已开发出一种在线浓缩方法，用于通过流场-流分级法（FFF）分析大分子和颗粒的稀悬浮液。这种方法称为逆流样品浓缩（OFSC），它使用两个相反的流股将样品聚焦到流场FFF通道入口附近的窄带中。已成功在1 mL体积流的FFF通道中加载、浓缩和分离了大体积样品（高达1 L）。实现了高达10⁵的浓缩因子。使用蛋白质和聚苯乙烯乳胶珠等单分散标准品的混合物证明了OFSC流场FFF的有效性。对于10 mL和典型的10 μL进样体积，保留时间、峰面积和分辨率相当。通过对河水胶体的分离证明了该方法在环境研究中的适用性。
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