Eremeeva M, Yu X, Raoult D
Unité des Rickettsies, Faculté de Médecine, Centre National de la Recherche Scientifique EP J0054, Marseille, France.
J Clin Microbiol. 1994 Mar;32(3):803-10. doi: 10.1128/jcm.32.3.803-810.1994.
Restriction fragment length polymorphism (RFLP) analysis of PCR-amplified genes was used to study spotted fever group (SFG) rickettsiae, extending the previous work of Regnery et al. (R.L. Regnery, C.L. Spruill, and B.D. Plikaytis, J. Bacteriol. 173:1576-1589, 1991). Twenty-six strains of SFG rickettsia were studied, including several recognized species which have never been studied (R. parkeri, R. helvetica, and R. japonica) as well as strains which are not currently classified. Two previously used primer pairs derived from the R. prowazekii citrate syntase gene and the R. rickettsii 190-kDa protein antigen gene were studied, as were primer pairs obtained from the R. rickettsii 120-kDa protein antigen gene. By using three amplifications and three enzyme digestions, it was possible to differentiate between almost all of the known SFG rickettsia species and to differentiate between several strains of the R. conorii complex. Two human pathogens, "R. africae" and the Israeli tick typhus rickettsia, were first separated by using BG-12 pair primer amplification and then RsaI restriction endonuclease digestion. The proposed simplified model of identification may be useful in studying the geographical distributions of SFG rickettsiae.
采用聚合酶链反应（PCR）扩增基因的限制性片段长度多态性（RFLP）分析方法对斑点热群（SFG）立克次体进行研究，拓展了Regnery等人之前的工作（R.L. Regnery、C.L. Spruill和B.D. Plikaytis，《细菌学杂志》173:1576 - 1589，1991）。研究了26株SFG立克次体菌株，包括几种从未被研究过的公认菌种（帕克立克次体、瑞士立克次体和日本立克次体）以及目前未分类的菌株。研究了之前使用的源自普氏立克次体柠檬酸合酶基因和立氏立克次体190 kDa蛋白抗原基因的两对引物，以及从立氏立克次体120 kDa蛋白抗原基因获得的引物对。通过三次扩增和三次酶切，可以区分几乎所有已知的SFG立克次体菌种，并区分康氏立克次体复合体的几个菌株。两种人类病原体，“非洲立克次体”和以色列蜱传斑疹伤寒立克次体，首先通过BG - 12对引物扩增，然后用RsaI限制性内切酶消化进行分离。所提出的简化鉴定模型可能有助于研究SFG立克次体的地理分布。
