Paolisso G, Tagliamonte M R, Rizzo M R, Manzella D, Gambardella A, Varricchio M
Department of Geriatric Medicine and Metabolic Diseases-II, University of Naples, Italy.
J Am Geriatr Soc. 1998 Jul;46(7):833-8. doi: 10.1111/j.1532-5415.1998.tb02716.x.
Our study aims at investigating the degree of oxidative stress in centenarians
Indices of oxidative stress (reaction products of malondialdehyde with thiobarbituric acid (TBARS) and lipid hydroperoxides (LPO)), and plasma concentrations of antioxidant defenses (plasma vitamin E and C concentrations and reduced/oxidized glutathione ratio (GSH/GSSG)) were determined.
Eighty-two subjects volunteered for the study. They were divided into three groups: (1) adults (<50 years of age, n=30); (2) aged subjects (70-99 years, n=30); (3) centenarians (age > or=100 years, n=22).
TBARS and LPO, plasma vitamin E and C concentrations, and plasma GSH/GSSG ratio were determined. Insulin action was assessed by euglycemic hyperinsulinemic glucose clamp.
TBARS (0.44+/-0.07 vs 0.31+/-.05 nmol malondialdehyde/mL plasma, P=.020) and LPO (0.36+/-0.05 vs 0.31+/-.04 micromol/L, P=.050) were lower in centenarians than in aged subjects. In contrast, plasma GSH/GSSG ratio (0.82+/-0.09 vs 1.17+/-.06, P=.010), vitamin C (72.3+/-4.6 vs 59.4+/-3.8 micromol/L P=.010), and vitamin E (29.1+/-2.2 vs 24.4+/-2.3 micromol/L P=.050) concentrations were more elevated in centenarians than in aged subjects. Differences in daily vegetable intake, in fasting plasma glucose and free fatty acid (FFA) concentrations, and in insulin action are significant determinants of degree of oxidative stress. A specific genetic background in centenarians might also provide a possible explanation.
The degree of oxidative stress is lower in healthy centenarians than in aged subjects.
我们的研究旨在调查百岁老人的氧化应激程度。
测定氧化应激指标（丙二醛与硫代巴比妥酸的反应产物（TBARS）和脂质氢过氧化物（LPO））以及抗氧化防御的血浆浓度（血浆维生素E和C浓度以及还原型/氧化型谷胱甘肽比率（GSH/GSSG））。
82名受试者自愿参与本研究。他们被分为三组：（1）成年人（<50岁，n = 30）；（2）老年人（70 - 99岁，n = 30）；（3）百岁老人（年龄≥100岁，n = 22）。
测定TBARS和LPO、血浆维生素E和C浓度以及血浆GSH/GSSG比率。通过正常血糖高胰岛素葡萄糖钳夹技术评估胰岛素作用。
百岁老人的TBARS（0.44±0.07对0.31±0.05 nmol丙二醛/毫升血浆，P = 0.020）和LPO（0.36±0.05对0.31±0.04微摩尔/升，P = 0.050）低于老年人。相反，百岁老人的血浆GSH/GSSG比率（0.82±0.09对1.17±0.06，P = 0.010）、维生素C（72.3±4.6对59.4±3.8微摩尔/升，P = 0.010）和维生素E（29.1±2.2对24.4±2.3微摩尔/升，P = 0.050）浓度高于老年人。每日蔬菜摄入量、空腹血糖和游离脂肪酸（FFA）浓度以及胰岛素作用的差异是氧化应激程度的重要决定因素。百岁老人特定的遗传背景也可能提供一种解释。
健康百岁老人的氧化应激程度低于老年人。
