PCAF组蛋白乙酰化酶复合物中的类组蛋白TAF

Histone-like TAFs within the PCAF histone acetylase complex.

作者信息

Ogryzko V V, Kotani T, Zhang X, Schiltz R L, Howard T, Yang X J, Howard B H, Qin J, Nakatani Y

机构信息

Laboratory of Molecular Growth Regulation, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.

出版信息

Cell. 1998 Jul 10;94(1):35-44. doi: 10.1016/s0092-8674(00)81219-2.

DOI:10.1016/s0092-8674(00)81219-2

PMID:9674425

Abstract

PCAF histone acetylase plays a role in regulation of transcription, cell cycle progression, and differentiation. Here, we show that PCAF is found in a complex consisting of more than 20 distinct polypeptides. Strikingly, some polypeptides are identical to TBP-associated factors (TAFs), which are subunits of TFIID. Like TFIID, histone fold-containing factors are present within the PCAF complex. The histone H3- and H2B-like subunits within the PCAF complex are identical to those within TFIID, namely, hTAF(II)31 and hTAF(II)20/15, respectively. The PCAF complex has a novel histone H4-like subunit with similarity to hTAF(II)80 that interacts with the histone H3-like domain of hTAF(II)31. Moreover, the PCAF complex has a novel subunit with WD40 repeats having a similarity to hTAF(II)100.

摘要

PCAF组蛋白乙酰转移酶在转录调控、细胞周期进程和分化过程中发挥作用。在此，我们发现PCAF存在于一个由20多种不同多肽组成的复合物中。令人惊讶的是，一些多肽与TBP相关因子（TAFs）相同，而TAFs是TFIID的亚基。与TFIID一样，PCAF复合物中也存在含组蛋白折叠的因子。PCAF复合物中的组蛋白H3样和H2B样亚基分别与TFIID中的亚基相同，即hTAF(II)31和hTAF(II)20/15。PCAF复合物有一个与hTAF(II)80相似的新型组蛋白H4样亚基，它与hTAF(II)31的组蛋白H3样结构域相互作用。此外，PCAF复合物还有一个与hTAF(II)100相似的具有WD40重复序列的新型亚基。