Santino I, Iori A, Sessa R, Sulli C, Favia G, Del Piano M
Institute of Microbiology, La Sapienza University, Rome, Italy.
FEMS Microbiol Lett. 1998 Jul 1;164(1):1-6. doi: 10.1111/j.1574-6968.1998.tb13059.x.
The aims of the present study were (a) to determine the presence of Ixodes ricinus in three different areas of the National Park of Abruzzo; (b) to search for the presence of Borrelia burgdorferi in the collected sample of Ixodes; (c) to determine the seroprevalence of B. burgdorferi antibodies and E. chaffeensis antibodies in inhabitants of the park and in park workers. The presence of B. burgdorferi in Ixodes was checked by PCR. For the detection of antibodies to B. burgdorferi all sera were assayed by ELISA as screening test and by Western blot as confirmatory test. For the detection of antibodies to E. chaffeensis all sera were assayed by IFA. Antibodies to B. burgdorferi were present in 9.1% of the park workers, 4.5% were confirmed positive by the IgG Western blot test. None of the inhabitants of the park was positive. Antibodies against E. chaffeensis were found in 4.5% of the park workers and 8% of the inhabitants of the park. The results obtained in the collecting of the ticks seem to show that the presence of I. ricinus in the park territory is rather discontinuous and small in number, therefore it is not epidemiologically significant for the transmission of B. burgdorferi sensu lato. Serological study for Ehrlichia revealed a high frequency of E. chaffeensis antibodies in the park inhabitants and a lower prevalence in the park workers.
（a）确定阿布鲁佐国家公园三个不同区域是否存在蓖麻硬蜱；（b）在采集的蓖麻硬蜱样本中寻找伯氏疏螺旋体的存在；（c）确定公园居民和公园工作人员中伯氏疏螺旋体抗体和查菲埃立克体抗体的血清阳性率。通过聚合酶链反应（PCR）检测蓖麻硬蜱中伯氏疏螺旋体的存在。对于伯氏疏螺旋体抗体的检测，所有血清均通过酶联免疫吸附测定（ELISA）作为筛查试验，并通过免疫印迹法作为确证试验进行检测。对于查菲埃立克体抗体的检测，所有血清均通过间接荧光抗体试验（IFA）进行检测。9.1%的公园工作人员存在伯氏疏螺旋体抗体，4.5%通过免疫球蛋白G（IgG）免疫印迹试验确认为阳性。公园居民均为阴性。4.5%的公园工作人员和8%的公园居民发现存在查菲埃立克体抗体。蜱虫采集结果似乎表明，公园区域内蓖麻硬蜱的存在相当不连续且数量较少，因此对于广义伯氏疏螺旋体的传播在流行病学上不具有重要意义。针对埃立克体的血清学研究显示，公园居民中查菲埃立克体抗体的频率较高，而公园工作人员中的患病率较低。
