a Department of Infectious Diseases , Iuliu Haţieganu University of Medicine and Pharmacy , Cluj-Napoca , Romania.
b Clinical Hospital of Infectious Diseases , Cluj-Napoca , Romania.
Infect Dis (Lond). 2017 Apr;49(4):277-285. doi: 10.1080/23744235.2016.1258488. Epub 2016 Nov 21.
The risk of developing Lyme borreliosis (LB) after the bite of a Borrelia (B.) burgdorferi sensu lato (s.l.) infected tick in Romania is unknown.
The present prospective study, performed in 2010-2011 in a hospital in Romania, has followed-up clinical and serological outcome of patients that presented with B. burgdorferi positive Ixodes (I.) ricinus bite. A second group of patients, including age, sex and residence-matched individuals bitten by B. burgdorferi negative ticks, was followed-up as a control group. The subjects' outcome was evaluated one year after the tick bite.
Forty-three out of 389 ticks detached from patients were positive by hbb Real-Time PCR (RT-PCR) for B. burgdorferi s.l. (mainly B. afzelii, but also B. garinii, B. burgdorferi sensu stricto, B. spielmanii/B. valaisiana and B. lusitaniae). Twenty patients bitten by B. burgdorferi positive ticks and twenty matched control patients returned for the one year follow-up. Two patients from the B. burgdorferi positive group developed clinical manifestations of acute LB (erythema migrans) and 5 patients seroconverted (two from the B. burgdorferi positive group and three from the B. burgdorferi negative group). Borrelia afzelii was identified in ticks collected from persons that developed erythema migrans (EM). Comparing the two groups of patients, no statistical significant differences were found regarding presence of clinical symptoms or seroconversion.
No outcome differences were found between the group of patients bitten by B. burgdorferi positive ticks and the group of patients bitten by B. burgdorferi negative ticks.
在罗马尼亚，被感染伯氏疏螺旋体（B. burgdorferi sensu lato，s.l.）的蜱虫叮咬后患上莱姆病（LB）的风险尚不清楚。
本前瞻性研究于 2010-2011 年在罗马尼亚的一家医院进行，对患有伯氏疏螺旋体阳性伊氏革蜱（I. ricinus）叮咬的患者的临床和血清学结果进行了随访。作为对照组，还对年龄、性别和居住环境相匹配的、被伯氏疏螺旋体阴性蜱虫叮咬的第二组患者进行了随访。在蜱虫叮咬一年后评估患者的结局。
从 389 名患者身上取下的 43 只蜱虫通过 hbb 实时聚合酶链反应（RT-PCR）检测到伯氏疏螺旋体 s.l.（主要为伯氏疏螺旋体 afzelii，但也有伯氏疏螺旋体 garinii、伯氏疏螺旋体 sensu stricto、伯氏疏螺旋体 spielmanii/B. valaisiana 和伯氏疏螺旋体 lusitaniae）阳性。20 名被伯氏疏螺旋体阳性蜱虫叮咬的患者和 20 名匹配的对照组患者返回进行一年的随访。伯氏疏螺旋体阳性组中有 2 名患者出现急性 LB（游走性红斑）的临床表现，5 名患者血清学转化（其中 2 名来自伯氏疏螺旋体阳性组，3 名来自伯氏疏螺旋体阴性组）。从出现游走性红斑的患者身上采集的蜱虫中鉴定出伯氏疏螺旋体 afzelii。比较两组患者，在出现临床症状或血清学转化方面未发现统计学显著差异。
在被伯氏疏螺旋体阳性蜱虫叮咬的患者组和被伯氏疏螺旋体阴性蜱虫叮咬的患者组之间，未发现结局差异。
