Burkett G, Frank L A
Department of Small Animal Clinical Sciences, College of Veterinary Medicine, University of Tennessee, Knoxville 37901, USA.
J Am Vet Med Assoc. 1998 Jul 15;213(2):232-4.
To determine whether exotoxin production by Staphylococcus intermedius is an important factor in recurrence of pyoderma in atopic dogs.
Prospective clinical study.
30 client-owned dogs (10 clinically normal dogs, 10 atopic dogs with recurrent pyoderma, and 10 dogs with a single episode of pyoderma).
Specimens for bacterial culturing were obtained from a pustule or papule of affected dogs or from skin of clinically normal dogs. Staphylococcus intermedius was isolated and processed. Supernatants were analyzed for staphylococcal exotoxins. Types of lesions and severity of pruritus were assessed for each dog.
Exotoxins A and C were detected in S intermedius isolated from 1 clinically normal dog. In the atopic group, exotoxin C was isolated from 1 dog, exotoxins A and C were isolated from 1 dog, and exotoxin C and toxic shock syndrome toxin-1 were isolated from 1 dog. Exotoxins were isolated from 2 dogs with a single episode of pyoderma (exotoxin C, 1 dog; exotoxins A and C, 1 dog). Lesion types and severity of pruritus varied greatly among dogs with pyoderma and were not associated with detection of exotoxin or type of exotoxin.
Exotoxins were identified from few isolates of S intermedius, and we did not observe an association between type of lesions or severity of pruritus and detection of exotoxins. Production of exotoxin by S intermedius does not play a role in the recurrent nature of pyoderma in atopic dogs or on type of lesion or severity of pruritus associated with pyoderma.
确定中间葡萄球菌产生的外毒素是否是特应性犬脓皮病复发的重要因素。
前瞻性临床研究。
30只客户拥有的犬（10只临床正常犬、10只患有复发性脓皮病的特应性犬和10只患有单次脓皮病发作的犬）。
从患病犬的脓疱或丘疹或临床正常犬的皮肤获取细菌培养标本。分离并处理中间葡萄球菌。分析上清液中的葡萄球菌外毒素。评估每只犬的病变类型和瘙痒严重程度。
从1只临床正常犬分离出的中间葡萄球菌中检测到外毒素A和C。在特应性组中，从1只犬分离出外毒素C，从1只犬分离出外毒素A和C，从1只犬分离出外毒素C和中毒性休克综合征毒素-1。从2只患有单次脓皮病发作的犬中分离出了外毒素（外毒素C，1只犬；外毒素A和C，1只犬）。患有脓皮病的犬之间的病变类型和瘙痒严重程度差异很大，且与外毒素的检测或外毒素类型无关。
从少数中间葡萄球菌分离株中鉴定出了外毒素，并且我们未观察到病变类型或瘙痒严重程度与外毒素检测之间的关联。中间葡萄球菌产生外毒素在特应性犬脓皮病的复发性质或与脓皮病相关的病变类型或瘙痒严重程度方面不起作用。
