来自球形芽孢杆菌的两种限制性内切酶。
Two restriction endonucleases from Bacillus globiggi.
作者信息
Pirrotta V
出版信息
Nucleic Acids Res. 1976 Jul;3(7):1747-60. doi: 10.1093/nar/3.7.1747.
Abstract
The sites of action of the restriction enzyme Bgl II on lambda DNA are mapped. This enzyme recognises the sequence 5' ...AGATCT...3' and makes staggered cuts producing sticky ends. In lambda DNA, the second A in this sequence is methylated about 50% of the time by a bacterial methylase absent in E. coli dam. In contrast to Bgl II, Bgl I makes many cuts in lambda DNA and produces 5' terminals which are not substrates for polynucleotide kinase.
摘要
已绘制出限制性内切酶Bgl II在λ噬菌体DNA上的作用位点。这种酶识别序列5'...AGATCT...3'，并进行交错切割产生黏性末端。在λ噬菌体DNA中，该序列中的第二个A大约有50%的时间会被大肠杆菌dam中不存在的一种细菌甲基化酶甲基化。与Bgl II不同，Bgl I在λ噬菌体DNA上有许多切割位点，并产生5'末端，这些末端不是多核苷酸激酶的底物。
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