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果蝇细胞中核RNA与细胞质RNA的关系。

Relationship between nuclear and cytoplasmic RNA in Drosophilia cells.

作者信息

Levy B, McCarthy B J

出版信息

Biochemistry. 1976 Jun 1;15(11):2415-9. doi: 10.1021/bi00656a026.

DOI:10.1021/bi00656a026
PMID:1276150
Abstract

Polyadenylated RNA was isolated from nuclei of cultured Drosophila cells, Schneider's line 2, and used as a template to synthesize a complementary DNA probe. Hybridization experiments were performed to study the relationship between nuclear and cytoplasmic RNA. About two-thirds of the nuclear polyadenylated RNA sequences exist in the cytoplasm. Experiments with fractionated cDNA probes demonstrated that RNA sequences that are frequent in the nucleus are also abundant in the cytoplasm. These findings are consistent with a precursor-product relationship in which some polyadenylated molecules in the nucleus are destined for the cytoplasm while other sequences are polyadenylated but not transferred.

摘要

从培养的果蝇细胞（施奈德2号线）的细胞核中分离出多聚腺苷酸化RNA，并将其用作模板来合成互补DNA探针。进行杂交实验以研究细胞核RNA和细胞质RNA之间的关系。大约三分之二的细胞核多聚腺苷酸化RNA序列存在于细胞质中。用分级分离的cDNA探针进行的实验表明，在细胞核中频繁出现的RNA序列在细胞质中也很丰富。这些发现与前体-产物关系一致，即细胞核中的一些多聚腺苷酸化分子 destined for the cytoplasm，而其他序列则被多聚腺苷酸化但未转移。 （原文中“destined for the cytoplasm”表述不太完整准确，完整准确的可能是“注定要进入细胞质” ）

相似文献

1
Relationship between nuclear and cytoplasmic RNA in Drosophilia cells.果蝇细胞中核RNA与细胞质RNA的关系。
Biochemistry. 1976 Jun 1;15(11):2415-9. doi: 10.1021/bi00656a026.
2
Diversity of sequences in total and polyadenylated nuclear RNA from Drosophila cells.果蝇细胞中总核RNA和聚腺苷酸化核RNA的序列多样性。
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Frequency distribution of pre-messenger RNA sequences in polyadenylated and non-polyadenylated nuclear RNA from Friend cells.来自弗瑞德（Friend）细胞的多聚腺苷酸化和非多聚腺苷酸化核RNA中前体信使RNA序列的频率分布。
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4
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Comparison of cytoplasmic and nuclear poly(A)-containing RNA sequences in Xenopus liver cells.非洲爪蟾肝细胞中细胞质和细胞核含多聚腺苷酸RNA序列的比较。
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Relative distribution of post-nuclear poly(A)-containing RNA abundance groups within the nuclear and post-nuclear polyadenylated and non-polyadenylated RNA populations of the lactating guinea-pig mammary gland.哺乳期豚鼠乳腺细胞核内及核后多聚腺苷酸化和非多聚腺苷酸化RNA群体中含核后多聚（A）RNA丰度组的相对分布。
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Chinese hamster polyadenylated messenger ribonucleic acid: relationship to non-polyadenylated sequences and relative conservation during messenger ribonucleic acid processing.中国仓鼠多聚腺苷酸化信使核糖核酸：与非多聚腺苷酸化序列的关系以及在信使核糖核酸加工过程中的相对保守性。
Mol Cell Biol. 1981 Feb;1(2):188-98. doi: 10.1128/mcb.1.2.188-198.1981.

引用本文的文献

1
A comparison of sequence complexity of nuclear and polysomal poly(A) RNA from different developmental stages ofXenopus laevis.非洲爪蟾不同发育阶段细胞核和多核糖体多聚腺苷酸RNA序列复杂性的比较。
Wilehm Roux Arch Dev Biol. 1980 Oct;188(3):187-193. doi: 10.1007/BF00849047.
2
Sequence complexity and diversity of polyadenylated RNA molecules of human normal resting, PHA-stimulated and leukemic lymphocytes.人类正常静息、PHA刺激的淋巴细胞及白血病淋巴细胞的聚腺苷酸化RNA分子的序列复杂性和多样性
Nucleic Acids Res. 1981 Dec 21;9(24):7013-32. doi: 10.1093/nar/9.24.7013.
3
Concentrations of individual RNA sequences in polyadenylated nuclear and cytoplasmic RNA populations of Drosophila cells.
果蝇细胞多聚腺苷酸化核RNA和细胞质RNA群体中单个RNA序列的浓度。
Nucleic Acids Res. 1980 Dec 20;8(24):6099-111. doi: 10.1093/nar/8.24.6099.
4
Gene control in eukaryotes and the c-value paradox "excess" DNA as an impediment to transcription of coding sequences.真核生物中的基因调控与C值悖论：“多余”的DNA对编码序列转录的阻碍
J Mol Evol. 1976 Dec 31;9(1):73-104. doi: 10.1007/BF01796124.
5
Sequences coding for proteins expressed in liver and for globin in poly(A)+ and poly(A)- RNA fractions from nuclei and cytoplasm of chicken immature red blood cells.编码鸡未成熟红细胞细胞核和细胞质中聚腺苷酸（poly(A)）阳性和聚腺苷酸阴性RNA组分中肝脏表达的蛋白质以及珠蛋白的序列。
Mol Biol Rep. 1978 Feb 28;4(1):39-44. doi: 10.1007/BF00775179.
6
Regulation of albumin synthesis in rat liver.大鼠肝脏中白蛋白合成的调节
Mol Biol Rep. 1979 May 31;5(1-2):71-8. doi: 10.1007/BF00777491.
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Frequency distribution of mRNA and pre-mRNA in growing and differentiated Friend cells.生长和分化的Friend细胞中mRNA和前体mRNA的频率分布。
Nucleic Acids Res. 1978 Aug;5(8):3013-32. doi: 10.1093/nar/5.8.3013.
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Use of molecular hybridization to purify and analyze albumin messenger RNA from rat liver.利用分子杂交技术从大鼠肝脏中纯化和分析白蛋白信使核糖核酸。
Proc Natl Acad Sci U S A. 1977 Oct;74(10):4346-50. doi: 10.1073/pnas.74.10.4346.