Elwing H
Department of General and Marine Microbiology, Göteborg University, Sweden.
Biomaterials. 1998 Mar;19(4-5):397-406. doi: 10.1016/s0142-9612(97)00112-9.
Ellipsometry is an optical surface-sensitive method for the investigation of various aspects of protein adsorption mainly at reflecting metal surfaces and ceramic surfaces. One interesting feature of the method is the possibility of detailed and accurate determination of real-time adsorption kinetics of proteins without labelling of the protein. It is also possible to detect protein adsorption with the use of antibodies that adsorb onto the antigen-coated surfaces and to detect antibodies by their adsorption behaviour with regard to antigen-coated surfaces. Compared to other solid phase methods such as enzyme linked immunosorbent assay (ELISA), immunofluorescence and radioimmunoassay, ellipsometry has the advantage of not involving any labelling of the reactant and it is a relatively inexpensive method to maintain. This review is a current report of 15 years of contributions in biomaterials and biochemical research. Special consideration has been given to biologically related surface phenomena such as protein conformation changes, protein displacement effects, early events in blood clotting and complement activation. Among the technical achievements given prominence are the wettability gradient method and the rational use of silicon as an experimental surface. It is clear that ellipsometry and related methods such as reflectometry and surface plasmon resonance (Biacore) are now being increasingly used in biomaterial research as well as in other areas of research.
椭圆偏振技术是一种光学表面敏感方法，主要用于研究蛋白质在反射金属表面和陶瓷表面吸附的各个方面。该方法的一个有趣特性是能够在不标记蛋白质的情况下详细且准确地测定蛋白质的实时吸附动力学。利用吸附在抗原包被表面的抗体来检测蛋白质吸附，以及通过抗体在抗原包被表面的吸附行为来检测抗体也是可行的。与其他固相方法如酶联免疫吸附测定（ELISA）、免疫荧光和放射免疫测定相比，椭圆偏振技术具有不涉及任何反应物标记的优点，并且维护成本相对较低。本综述是对15年来在生物材料和生化研究方面贡献的一份最新报告。特别关注了与生物学相关的表面现象，如蛋白质构象变化、蛋白质置换效应、血液凝固和补体激活的早期事件。在突出的技术成就中包括润湿性梯度方法以及合理使用硅作为实验表面。显然，椭圆偏振技术以及相关方法如反射测量法和表面等离子体共振（Biacore）现在在生物材料研究以及其他研究领域中越来越多地被使用。
