Laufer H, Biggers W J, Ahl J S
Department of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut, 06269, USA.
Gen Comp Endocrinol. 1998 Aug;111(2):113-8. doi: 10.1006/gcen.1998.7109.
Stimulation of ovarian maturation in the red swamp crayfish Procambarus clarkii was accomplished in three different trials by administration of methylfarnesoate (MF). After 30 days of treatment, the ovaries of prereproductive females were 2- to 10-fold larger and in later stages of vitellogenesis than those of the controls. Similar and statistically significant results were observed in a second 30-day trial, which was begun during the middle of the vitellogenic cycle. In this experiment the ovaries of the controls and the treated groups were in late vitellogenesis, and the oocyte diameters of both groups were similar, suggesting that MF stimulated more oocytes to mature. In a third experiment of 60 days duration, the ovary was again enlarged in the treated animals. In addition, MF levels in the hemolymph of treated females undergoing vitellogenesis were found to be twice as great as those of the controls. We conclude that exogenous MF can stimulate and enhance ovarian maturation. These experiments strongly support the concept that MF acts as a gondatropin in crustaceans.
在三项不同的试验中，通过施用法尼酸甲酯（MF）实现了对克氏原螯虾卵巢成熟的刺激。经过30天的处理后，生殖前期雌性的卵巢比对照组的大2至10倍，且处于卵黄发生的后期阶段。在卵黄发生周期中期开始的第二项为期30天的试验中，观察到了类似且具有统计学意义的结果。在该实验中，对照组和处理组的卵巢都处于卵黄发生后期，且两组的卵母细胞直径相似，这表明MF刺激了更多的卵母细胞成熟。在为期60天的第三项实验中，处理组动物的卵巢再次增大。此外，发现处于卵黄发生期的处理组雌性血淋巴中的MF水平是对照组的两倍。我们得出结论，外源性MF可以刺激并增强卵巢成熟。这些实验有力地支持了MF在甲壳类动物中作为促性腺激素起作用的概念。
