Yu F Y, Chu F S
University of Wisconsin-Madison, Food Research Institute 53706, USA.
J AOAC Int. 1998 Jul-Aug;81(4):749-56.
Use of a direct competitive enzyme-linked immunosorbent assay (ELISA) as a postcolumn monitoring system after liquid chromatography (LC) is described for analysis of different fumonisin analogs. Without cleanup and derivatization, sample extracts are directly injected into a C18 reversed-phase column and then subjected to LC. Fractions (0.5 mL each) are collected and then analyzed by ELISA. LC using a water-methanol gradient separated the 3 major fumonisins FmB1, FmB2, and FmB3, and as low as 0.1 ng FmB1 could be detected. Recovery of FmB1 added to ground corn (100-1000 ng/g) and then extracted with CH3CN-H2O (1 + 1, v/v) was 78.8%. Analysis of fumonisins in one starch and 14 naturally contaminated corn samples showed that FmB1 was the major fumonisin. Ten samples also were contaminated with FmB2, but only 2 samples were contaminated with FmB3. The method also was used to analyze extracts from cultures of 3 Alternaria alternata (AAL) strains. Both FmB1 and the AAL toxin TA were detected in the culture extracts, and their amounts varied considerably with the cultures tested.
本文描述了一种直接竞争酶联免疫吸附测定法（ELISA）作为液相色谱（LC）后柱监测系统，用于分析不同伏马菌素类似物。无需净化和衍生化，样品提取物直接注入C18反相柱，然后进行LC分析。收集馏分（每份0.5 mL），然后通过ELISA进行分析。使用水-甲醇梯度的LC分离出3种主要伏马菌素FmB1、FmB2和FmB3，最低可检测到0.1 ng FmB1。添加到磨碎玉米（100 - 1000 ng/g）中然后用CH3CN-H2O（1 + 1，v/v）提取的FmB1回收率为78.8%。对一份淀粉和14份天然污染玉米样品中的伏马菌素分析表明，FmB1是主要的伏马菌素。10个样品还被FmB2污染，但只有2个样品被FmB3污染。该方法还用于分析3株链格孢（AAL）菌株培养物的提取物。在培养物提取物中检测到FmB1和AAL毒素TA，其含量随测试的培养物有很大差异。
