Desreumaux P, Delaporte E, Colombel J F, Capron M, Cortot A, Janin A
Laboratoire de Recherche sur les Maladies Inflammatoires Intestinales, Centre Hospitalier Universitaire (CHU), Lille, France.
Clin Immunol Immunopathol. 1998 Jul;88(1):14-21. doi: 10.1006/clin.1997.4494.
Celiac disease (CD) and dermatitis herpetiformis (DH) are gluten-sensitive diseases with different clinical features that can initiate similar intestinal changes. The flat-destructive stage corresponds to severe lesions involving activated T-cells. However, other inflammatory cells such as eosinophils are also abundant. The mechanisms for the intestinal recruitment of eosinophils in patients with CD and DH remain unknown. Eosinophil recruitment and activation are induced in vitro by three main cytokines: interleukin-3 (IL-3), interleukin-5 (IL-5), and granulocyte-macrophage colony-stimulating factor (GM-CSF). In this study, IL-3, IL-5, and GM-CSF were detected by immunohistochemistry in all patients with CD and DH but not in the control group. By ultrastructural immunogold staining, these three cytokines had the same subcellular localization in the granule matrix of eosinophils. This result suggests that eosinophils may be involved in the immune response at the flat-destructive stage of both CD and DH.
乳糜泻（CD）和疱疹样皮炎（DH）是具有不同临床特征的麸质敏感疾病，可引发相似的肠道变化。扁平破坏性阶段对应于涉及活化T细胞的严重病变。然而，其他炎症细胞如嗜酸性粒细胞也很丰富。CD和DH患者肠道中嗜酸性粒细胞募集的机制尚不清楚。嗜酸性粒细胞的募集和激活在体外由三种主要细胞因子诱导：白细胞介素-3（IL-3）、白细胞介素-5（IL-5）和粒细胞-巨噬细胞集落刺激因子（GM-CSF）。在本研究中，通过免疫组织化学在所有CD和DH患者中检测到IL-3、IL-5和GM-CSF，但在对照组中未检测到。通过超微结构免疫金染色，这三种细胞因子在嗜酸性粒细胞的颗粒基质中具有相同的亚细胞定位。这一结果表明，嗜酸性粒细胞可能参与了CD和DH扁平破坏性阶段的免疫反应。
