[检测混合血清中的HIV抗体]
[Detection of HIV antibodies in pooled sera].
作者信息
López G, Silva C, Benítez J
机构信息
Centro de Investigaciones Científicas de la Defensa Civil.
出版信息
Rev Cubana Med Trop. 1997;49(1):32-7.
The use of sera mixtures for the detection of HIV antibodies was studied in a population of low prevalence (0.006%), using the diagnosis systems of national production: Recombinant HIV UMELISA and RECHIV 1 + 2. The systems were evaluated with individual samples and mixtures of 5 and 10 sera from healthy blood donors and from HIV-1 and/or HIV-2 positive and undetermined persons. It was observed that the utilization of mixtures of 10 sera is not feasible; however, the use of mixtures of 5 affects neither the specificity nor the sensitivity of the systems studied.
使用国产诊断系统重组HIV酶联免疫吸附测定法（Recombinant HIV UMELISA）和RECHIV 1 + 2，在低流行率（0.006%）人群中研究了血清混合物用于检测HIV抗体的情况。使用来自健康献血者、HIV-1和/或HIV-2阳性及未确诊者的个体样本以及5份和10份血清的混合物对这些系统进行了评估。观察到使用10份血清的混合物不可行；然而，使用5份血清的混合物对所研究系统的特异性和敏感性均无影响。
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