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用于快速检测62种病原菌的革兰氏类型特异性广谱PCR扩增

Gram type-specific broad-range PCR amplification for rapid detection of 62 pathogenic bacteria.

作者信息

Klausegger A, Hell M, Berger A, Zinober K, Baier S, Jones N, Sperl W, Kofler B

机构信息

Children's Hospital, General Hospital Salzburg, Salzburg, Austria.

出版信息

J Clin Microbiol. 1999 Feb;37(2):464-6. doi: 10.1128/JCM.37.2.464-466.1999.

DOI:10.1128/JCM.37.2.464-466.1999
PMID:9889245
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC84344/
Abstract

Broad-range PCR has proven to be useful for the detection of bacteria. A set of broad-range PCR primers directed against conserved regions in the 16S rRNA gene was designed to specifically amplify either gram-positive or gram-negative bacteria. The gram type-specific broad-range PCR correctly classified all 62 pathogenic species tested.

摘要

广谱聚合酶链反应（Broad-range PCR）已被证明对细菌检测有用。设计了一组针对16S核糖体RNA（rRNA）基因保守区域的广谱PCR引物，以特异性扩增革兰氏阳性或革兰氏阴性细菌。革兰氏类型特异性广谱PCR对所有62种测试的致病物种进行了正确分类。

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