来自澳大利亚、美国和南非的12种甘蔗花叶马铃薯Y病毒分离株外壳蛋白编码区的序列多样性。

Sequence diversity in the coat protein coding region of twelve sugarcane mosaic potyvirus isolates from Australia, USA and South Africa.

作者信息

Handley J A, Smith G R, Dale J L, Harding R M

机构信息

Centre for Molecular Biotechnology, School of Life Sciences, Queensland University of Technology, Brisbane, Australia.

出版信息

Arch Virol. 1998;143(6):1145-53. doi: 10.1007/s007050050362.

DOI:10.1007/s007050050362

PMID:9687871

Abstract

We have sequenced the coat protein (CP) coding region of 11 field isolates of SCMV from Australia, USA and South Africa. The differences between the nucleotide sequences of the isolates was 0.2 to 4.1% and the encoded amino acid sequences differed by 0.0 to 3.5%. Phylogenetic analysis of the CP coding sequences of the SCMV isolates and the related potyviruses SCMV-MDB, JGMV, SrMV, MDMV-A and PVY showed that the SCMV isolates formed a tightly clustered group, with SCMV-MDB forming a separate branch. This indicated that (i) the SCMV isolates are of one strain (SCMV-A) and not geographically distinct species and (ii) SCMV-MDB is clearly distinct, and may represent another potyvirus species.

摘要

我们对来自澳大利亚、美国和南非的11株甘蔗花叶病毒（SCMV）田间分离株的外壳蛋白（CP）编码区进行了测序。这些分离株的核苷酸序列差异为0.2%至4.1%，编码的氨基酸序列差异为0.0%至3.5%。对SCMV分离株以及相关马铃薯Y病毒属病毒SCMV-MDB、玉米线条病毒（JGMV）、高粱花叶病毒（SrMV）、甘蔗花叶病毒MDMV-A株（MDMV-A）和马铃薯Y病毒（PVY）的CP编码序列进行系统发育分析表明，SCMV分离株形成了一个紧密聚类的组，而SCMV-MDB形成了一个单独的分支。这表明：（i）SCMV分离株属于一个毒株（SCMV-A），而非地理上不同的物种；（ii）SCMV-MDB明显不同，可能代表另一种马铃薯Y病毒属病毒。