Matsuda Y, Nakagomi O, Offit P A
Animal Facilities for Experimental Medicine, Akita University School of Medicine, Japan.
Arch Virol. 1990;115(3-4):199-207. doi: 10.1007/BF01310530.
Cross neutralization tests with a panel of rotavirus strains representing previously described nine VP7 (G) serotypes revealed that bovine rotavirus strain KK-3, a prototype Japanese bovine serotype 2, belonged to a new serotype (G10), confirming and extending the recent report of Snodgrass et al. [J. Clin. Microbiol. 28: 504-507 (1990)] which showed that hyperimmune serum to the KK-3 strain neutralized the B223 strain, a proposed type strain of G10. Further antigenic characteristics of the KK-3 strain, as well as the 0510 strain (a G6 strain isolated in Japan), were examined in terms of their VP4 (P) specificity. For the characterization of P types, we employed genetic reassortants that possess VP4 gene for UK and VP7 gene for D (G1), VP4 gene for NCDV and VP7 gene for SA11 (G3), or VP4 gene for SA11 and VP7 gene for NCDV (G6) in the plaque reduction neutralization assay with hyperimmune sera against these two Japanese strains and the prototype bovine rotavirus NCDV strain. While the 0510 strain had UK-like P and NCDV-like G types, the KK-3 strain had a distinct set of P and G types. Thus, at least three P types (NCDV-, UK-, and KK-3-like) and two G types (G6 and G10) are present among bovine rotavirus strains.
用一组代表先前描述的9种VP7（G）血清型的轮状病毒株进行交叉中和试验，结果显示，日本牛血清型2的原型牛轮状病毒株KK-3属于一种新的血清型（G10），这证实并扩展了Snodgrass等人[《临床微生物学杂志》28：504 - 507（1990）]最近的报告，该报告表明，针对KK-3株的超免疫血清可中和G10的提议原型株B223株。根据VP4（P）特异性，对KK-3株以及0510株（在日本分离的G6株）的进一步抗原特性进行了研究。为了鉴定P型，我们在空斑减少中和试验中使用了基因重配体，这些重配体分别具有UK的VP4基因和D（G1）的VP7基因、NCDV的VP4基因和SA11（G3）的VP7基因，或SA11的VP4基因和NCDV（G6）的VP7基因，并使用针对这两种日本株和原型牛轮状病毒NCDV株的超免疫血清。虽然0510株具有类似UK的P型和类似NCDV的G型，但KK-3株具有独特的P型和G型组合。因此，牛轮状病毒株中至少存在三种P型（类似NCDV、UK和KK-3）和两种G型（G6和G10）。
