Vo L T, Papworth G D, Delaney P M, Barkla D H, King R G
Department of Pharmacology, Monash University, Clayton, Victoria, Australia.
Burns. 1998 Jun;24(4):319-24. doi: 10.1016/s0305-4179(98)00028-x.
Burn injury causes vascular thrombosis and occlusion by thermal damage to the vascular network in the dermis. In this study, fibre optic confocal imaging (FOCI) and laser doppler flowmetry were used to detect changes in vascular morphology and local dermal blood flux over 4 h, in three defined zones after a thermal burn (50 degrees C, 20 s duration, 3 mm in diameter) was induced on fully anaesthetised hairless mice. FITC-dextran (i.v.) was used to enable FOCI of vascular morphology including three-dimensional imaging of the burn site and its surrounding areas. Samples of the affected areas were collected for conventional histology, including Masson's trichrome. There was vascular damage in the zone of coagulation which showed no change during the 4 h period. The zone of stasis showed an initial reduction in blood flux and confocal imaging of the area indicated significant vessel leakage during the first 2 h which later improved. The zone of hyperaemia showed an initial increase in total blood flux and confocal imaging of the area showed initial blood vessel dilatation. This study demonstrates that FOCI is a useful non-invasive tool in the assessment of vascular changes in thermal burns in vivo, and compares the findings of FOCI with those from laser doppler flowmetry and histology.
烧伤通过对真皮血管网络的热损伤导致血管血栓形成和闭塞。在本研究中，利用光纤共聚焦成像（FOCI）和激光多普勒血流仪，在完全麻醉的无毛小鼠身上诱导产生热烧伤（50摄氏度，持续20秒，直径3毫米）后，对三个明确区域在4小时内的血管形态变化和局部真皮血流量进行检测。静脉注射异硫氰酸荧光素标记葡聚糖（FITC - dextran），以实现对血管形态的FOCI检测，包括对烧伤部位及其周围区域的三维成像。采集受影响区域的样本进行常规组织学检查，包括马松三色染色。凝固区存在血管损伤，在4小时内无变化。淤血区显示血流量最初减少，该区域的共聚焦成像表明在最初2小时内有明显的血管渗漏，随后有所改善。充血区显示总血流量最初增加，该区域的共聚焦成像显示血管最初扩张。本研究表明，FOCI是评估体内热烧伤血管变化的一种有用的非侵入性工具，并将FOCI的结果与激光多普勒血流仪和组织学的结果进行了比较。
