Raber T, Gorontzy T, Kleinschmidt M, Steinbach K, Blotevogel K H
Carl von Ossietzky Universität Oldenburg, Fachbereich Biologie, AG Mikrobiologie/Rüstungsaltlasten, Postfach 2503, 26111 Oldenburg, Germany.
Curr Microbiol. 1998 Sep;37(3):172-6. doi: 10.1007/s002849900359.
The ability of the strictly anaerobic sulfate-reducing bacterium Desulfobacula toluolica (strain Tol2) to cometabolically degrade p-toluidine (p-methylaniline) while using toluene as the primary source of carbon and energy has been studied. This organism has been shown to modify and degrade toluidine in dense cell suspensions when no other source of carbon and energy is added. The metabolism led to the formation of a variety of metabolites. From these metabolites a biphenyl-like compound as well as phenylacetic acid have been identified by means of HPLC/MS techniques. The probable conversion of p-toluidine to p-aminophenylacetic acid and phenylacetic acid as dead end products suggested that this organism initiates p-toluidine degradation by the carboxylation of the methyl group. If this could be validated in further experiments, it would be the first time that a toluidine was carboxylated at the methyl moiety by an anaerobic, sulfate-reducing bacterium.
已对严格厌氧的硫酸盐还原菌甲苯脱硫杆菌（菌株Tol2）在以甲苯作为主要碳源和能源时共代谢降解对甲苯胺（对甲基苯胺）的能力进行了研究。当不添加其他碳源和能源时，该微生物已被证明能在高密度细胞悬液中修饰和降解甲苯胺。这种代谢导致形成了多种代谢产物。通过高效液相色谱/质谱技术已从这些代谢产物中鉴定出一种联苯样化合物以及苯乙酸。对甲苯胺可能转化为对氨基苯乙酸和苯乙酸作为终产物，这表明该微生物通过甲基的羧化作用启动对甲苯胺的降解。如果这能在进一步实验中得到验证，这将是首次有厌氧硫酸盐还原菌使甲苯胺在甲基部分发生羧化反应。
