Veerman E C, Go K G, Molenaar W M, Amerongen A V, Vissink A
Department of Oral Biochemistry, Free University of Amsterdam, The Netherlands.
Acta Neurochir (Wien). 1998;140(4):303-6; discussion 306-7. doi: 10.1007/s007010050102.
Colloid cysts of the third ventricle have been investigated by chemical characterization of the cyst contents using ELISA with monoclonal antibodies for certain carbohydrate epitopes as well as a polyclonal antiserum against peptide domains, and immunohistochemistry on the cyst wall using the same antibodies. Furthermore, the carbohydrate composition of one sample has been determined after gel filtration. The cyst contents reacted strongly with the monoclonal antibody for the sulfo-Lewis epitope, and with the antimucin polyclonal antiserum. In one case the cyst fluid exhibited a blood group A antigen. A sample of cyst wall obtained by biopsy showed strong immunoreactivity against sulfo-Lewis antigen, and the sialo-Lewis antigen. The presence of the S atom with its high atomic number relative to that of C, H, and O atoms, may contribute to the high density appearance of colloid cysts on CT-scans. The sulfo-Lewis and sialo-Lewis carbohydrate epitopes are known as ligands for selectins, involved in inflammatory processes, and may well account for the aseptic meningeal reaction that may follow spilling of cyst contents during operative evacuation. The carbohydrate epitopes exhibited by colloid cysts and their contents, have also been reported for the mucins of salivary glands, uterine cervix, gall bladder and colon, and therefore, are not inconsistent with the assumption of an endodermal origin of colloid cysts.
通过使用针对某些碳水化合物表位的单克隆抗体进行酶联免疫吸附测定（ELISA）以及针对肽结构域的多克隆抗血清，对第三脑室的胶体囊肿内容物进行化学表征，并使用相同抗体对囊肿壁进行免疫组织化学研究，从而对第三脑室的胶体囊肿展开了研究。此外，在凝胶过滤后测定了一个样本的碳水化合物组成。囊肿内容物与针对硫代路易斯表位的单克隆抗体以及抗粘蛋白多克隆抗血清发生强烈反应。在一个病例中，囊液呈现出血型A抗原。通过活检获取的囊肿壁样本对硫代路易斯抗原和唾液酸化路易斯抗原有强烈的免疫反应性。相对于碳、氢和氧原子，具有高原子序数的硫原子的存在，可能导致胶体囊肿在CT扫描上呈现高密度影像。硫代路易斯和唾液酸化路易斯碳水化合物表位是已知的选择素配体，参与炎症过程，很可能解释了手术排空囊肿内容物时可能伴随的无菌性脑膜反应。胶体囊肿及其内容物所呈现的碳水化合物表位，在唾液腺、子宫颈、胆囊和结肠的粘蛋白中也有报道，因此，这与胶体囊肿起源于内胚层的假设并不矛盾。
