Dubowitz D J, Chen D Y, Atkinson D J, Grieve K L, Gillikin B, Bradley W G, Andersen R A
Division of Biology, California Institute of Technology, Pasadena, CA 91125, USA.
Neuroreport. 1998 Jul 13;9(10):2213-8. doi: 10.1097/00001756-199807130-00012.
The ability to use fMRI in a monkey model would bridge the gap between the fMRI demonstration of cerebral activation in humans and the cumulative wealth of monkey data on the functional organization of the brain from single electrode mapping, radioisotope and histology studies. We report a new technique for fMRI in an awake co-operative rhesus macaque (Macaca mulatta) in a conventional clinical 1.5T MR scanner and present the first fMRI images from a macaque. Good resolution, signal-to-noise ratio and BOLD response (2.6-4.6%) have been achieved using the manufacturer's standard volume knee coil. T1 values of macaque gray and white matter (1490 ms, 1010 ms respectively) are higher than human brain, whereas T2 values are lower (55 ms, 48 ms respectively). An MR-compatible design for restraining the monkey is also described, along with a suitable EPI sequence for BOLD images, optimized for monkey T2, with voxel sizes from 29 to 61 microl, and MPRAGE sequence for anatomical studies with 0.8 mm isotropic resolution, optimized for monkey T1.
在猴子模型中使用功能磁共振成像（fMRI）的能力，将弥合人类大脑激活的fMRI演示与来自单电极映射、放射性同位素和组织学研究的关于大脑功能组织的大量猴子数据之间的差距。我们报告了一种在传统临床1.5T磁共振成像（MR）扫描仪中对清醒合作的恒河猴（猕猴）进行fMRI的新技术，并展示了来自猕猴的首张fMRI图像。使用制造商的标准容积膝关节线圈已实现了良好的分辨率、信噪比和血氧水平依赖（BOLD）反应（2.6 - 4.6%）。猕猴灰质和白质的T1值（分别为1490毫秒和1010毫秒）高于人类大脑，而T2值较低（分别为55毫秒和48毫秒）。还描述了一种用于约束猴子的磁共振兼容设计，以及适用于BOLD图像的合适回波平面成像（EPI）序列，该序列针对猕猴T2进行了优化，体素大小为29至61微升，还有用于解剖学研究的磁化准备快速梯度回波（MPRAGE）序列，其各向同性分辨率为0.8毫米，针对猕猴T1进行了优化。
