Mooney M P, Siegel M I, Burrows A M, Smith T D, Losken H W, Dechant J, Cooper G, Fellows-Mayle W, Kapucu M R, Kapucu L O
Department of Anatomy and Histology, University of Pittsburgh, PA 15261, USA.
Childs Nerv Syst. 1998 Jun;14(6):247-55. doi: 10.1007/s003810050220.
This two-part study reviews data from a recently developed colony of New Zealand white rabbits with familial, nonsyndromic unilateral coronal suture synostosis, and this second part presents neuropathological findings and age-related changes in intracranial volume (ICV) and intracranial pressure (ICP) in 106 normal rabbits and 56 craniosynostotic rabbits from this colony. Brain morphology and anteroposterior length were described in 44 rabbit fetuses and perinates (27 normal; 17 synostosed). Middle meningeal artery patterns were qualitatively assessed from 2-D PCC MRI VENC scans and endocranial tracings from 15, 126-day-old rabbits (8 normal, 7 rabbits with unicoronal synostosis). Brain metabolism was evaluated by assessing 18F-FDG uptake with high-resolution PET scanning in 7, 25-day-old rabbits (3 normal, 4 with unicoronal or bicoronal synostosis). Intracranial contents and ICV were assessed using 3-D CT scanning of the skulls of 30 rabbits (20 normal,10 with unicoronal synostosis) at 42 and 126 days of age. Serial ICP data were collected from 66 rabbits (49 normal; 17 with unicoronal synostosis) at 25 and 42 days of age. ICP was assessed in the epidural space using a Codman NeuroMonitor microsensor transducer. Results revealed that cerebral cortex morphology was similar between normal and synostosed fetuses around the time of synostosis. Significantly (P<0.05) decreased A-P cerebral hemisphere growth rates and asymmetrical cortical remodeling were noted with increasing age in synostotic rabbits. In addition, rabbits with unicoronal suture synostosis exhibited asymmetrical middle meningeal artery patterns, decreased and asymmetrical brain metabolism, a "beaten-copper" intracranial appearance, significantly (P<0.05) decreased ICV, and significantly (P<0.01) elevated ICP compared with normal control rabbits. The advantages and disadvantages of these rabbits as a model for human familial, nonsyndromic unicoronal suture synostosis are discussed, especially in light of recent clinical neuropathological, ICV, and ICP findings recorded in human craniosynostotic studies.
这项分为两部分的研究回顾了来自一个最近培育的患有家族性、非综合征性单侧冠状缝早闭的新西兰白兔群体的数据，第二部分呈现了106只正常兔子和56只来自该群体的颅缝早闭兔子的神经病理学发现以及颅内体积（ICV）和颅内压（ICP）的年龄相关变化。描述了44只兔胎儿和围产期动物（27只正常；17只患有缝早闭）的脑形态和前后长度。通过二维相位对比造影磁共振成像（PCC MRI）VENC扫描和15只126日龄兔子（8只正常，7只单冠状缝早闭）的颅内膜描记图对脑膜中动脉模式进行了定性评估。通过在7只25日龄兔子（3只正常，4只单冠状或双冠状缝早闭）中使用高分辨率正电子发射断层扫描（PET）评估18F-氟代脱氧葡萄糖（18F-FDG）摄取来评价脑代谢。在30只兔子（20只正常，10只单冠状缝早闭）42日龄和126日龄时，使用颅骨的三维CT扫描评估颅内内容物和ICV。在66只兔子（49只正常；17只单冠状缝早闭）25日龄和42日龄时收集连续的ICP数据。使用Codman神经监测微传感器换能器在硬膜外间隙评估ICP。结果显示，在缝早闭时，正常和患有缝早闭的胎儿之间的大脑皮质形态相似。在患有缝早闭的兔子中，随着年龄增长，观察到显著（P<0.05）降低的大脑半球前后生长速率和不对称的皮质重塑。此外，与正常对照兔子相比，单冠状缝早闭的兔子表现出不对称的脑膜中动脉模式、降低和不对称的脑代谢、“铜箔样”颅内外观、显著（P<0.05）降低的ICV以及显著（P<0.01）升高的ICP。讨论了这些兔子作为人类家族性、非综合征性单冠状缝早闭模型的优缺点，特别是根据人类颅缝早闭研究中最近记录的临床神经病理学、ICV和ICP发现进行讨论。
