Ali M, Angelo-Khattar M, Thulesius L, Fareed A, Thulesius O
Department of Biochemistry, Faculty of Science, Kuwait University, Kuwait.
Urol Res. 1998;26(3):171-4. doi: 10.1007/s002400050042.
The purpose of this study was to investigate the site of production (urothelium/smooth muscle) and quantitative release of prostanoids in the ureter. Quantitative analysis of prostacyclin (PGI2) was by its metabolite, 6-keto PGFalpha, thromboxane (TXB2) and prostaglandin F2alpha. Synthesis by radiometry and radioimmunoassay was performed in vitro in sheep ureter specimens before and after removal of the inner epithelial layer and after addition of indomethacin. The major prostanoids present in the ureter were PGI2 and TXB2; PGI2 was quantitatively the largest component. Removal of the thin inner epithelial layer (urothelium) reduced mostly PGI2; addition of arachidonic acid significantly augmented PGI2 only in ureters with intact urothelium but did not alter TXB2 levels. The main source of prostanoid synthesis (PGI2) of the ureter is to be found in the urothelium. The functional role of the prostanoids may be related to coordination of peristalsis.
本研究的目的是调查输尿管中前列腺素的产生部位（尿路上皮/平滑肌）及其定量释放情况。前列环素（PGI2）通过其代谢产物6-酮-PGFα、血栓素（TXB2）和前列腺素F2α进行定量分析。在去除内层上皮后以及添加吲哚美辛后，通过放射测定法和放射免疫分析法在绵羊输尿管标本中进行体外合成。输尿管中存在的主要前列腺素是PGI2和TXB2；PGI2在数量上是最大的成分。去除薄的内层上皮（尿路上皮）主要降低了PGI2；添加花生四烯酸仅在尿路上皮完整的输尿管中显著增加了PGI2，但未改变TXB2水平。输尿管中前列腺素合成（PGI2）的主要来源存在于尿路上皮中。前列腺素的功能作用可能与蠕动的协调有关。
