Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S
Kato Cytoprotein Network Project, ERATO, Japan Science and Technology Corporation (JST), Sagami Chemical Research Center, Sagamihara, Kanagawa 229-0012, Japan.
Genes Dev. 1998 Aug 1;12(15):2263-8. doi: 10.1101/gad.12.15.2263.
NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an E1 (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino- and carboxy-terminal regions of E1 and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.
NEDD8是一种类泛素（Ub）蛋白。在此我们报告一种由NEDD8介导修饰的新型泛素化相关途径。NEDD8由一种类似E1（泛素激活酶）的复合物激活，该复合物由APP - BP1和hUba3组成，它们分别与E1的氨基末端和羧基末端区域具有高度同源性，然后与hUbc12（酵母泛素结合酶Ubc12p的人类同源物）相连。发现NEDD8修饰的主要靶蛋白是Hs - cullin - 4A（Cul - 4A），它是人类cullin/Cdc53蛋白家族的成员，作为多功能泛素 - 蛋白连接酶E3复合物的重要组成部分，在泛素介导的蛋白水解中起关键作用。
