一种针对cullin-4A的新型NEDD8连接系统。
A new NEDD8-ligating system for cullin-4A.
作者信息
Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S
机构信息
Kato Cytoprotein Network Project, ERATO, Japan Science and Technology Corporation (JST), Sagami Chemical Research Center, Sagamihara, Kanagawa 229-0012, Japan.
出版信息
Genes Dev. 1998 Aug 1;12(15):2263-8. doi: 10.1101/gad.12.15.2263.
Abstract
NEDD8 is a ubiquitin (Ub)-like protein. Here we report a novel ubiquitinylation-related pathway for modification by NEDD8. NEDD8 was activated by an E1 (Ub-activating enzyme)-like complex, consisting of APP-BP1 and hUba3 with high respective homologies to the amino- and carboxy-terminal regions of E1 and then linked to hUbc12 (a human homolog of yeast Ub-conjugating enzyme Ubc12p). The major target protein modified by NEDD8 was found to be Hs-cullin-4A (Cul-4A), a member of the family of human cullin/Cdc53 proteins functioning as an essential component of a multifunctional Ub-protein ligase E3 complex that has a critical role in Ub-mediated proteolysis.
摘要
NEDD8是一种类泛素（Ub）蛋白。在此我们报告一种由NEDD8介导修饰的新型泛素化相关途径。NEDD8由一种类似E1（泛素激活酶）的复合物激活，该复合物由APP - BP1和hUba3组成，它们分别与E1的氨基末端和羧基末端区域具有高度同源性，然后与hUbc12（酵母泛素结合酶Ubc12p的人类同源物）相连。发现NEDD8修饰的主要靶蛋白是Hs - cullin - 4A（Cul - 4A），它是人类cullin/Cdc53蛋白家族的成员，作为多功能泛素 - 蛋白连接酶E3复合物的重要组成部分，在泛素介导的蛋白水解中起关键作用。
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本文引用的文献
1
SUMO-1: Ubiquitin gains weight.SUMO-1：泛素增加体重。
Trends Cell Biol. 1997 Oct;7(10):408-13. doi: 10.1016/S0962-8924(97)01132-X.
2
A novel protein modification pathway related to the ubiquitin system.一种与泛素系统相关的新型蛋白质修饰途径。
EMBO J. 1998 Apr 15;17(8):2208-14. doi: 10.1093/emboj/17.8.2208.
3
Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex.泛素相关蛋白rub1p对酵母Cdc53p的修饰影响SCF Cdc4复合物的功能。
Genes Dev. 1998 Apr 1;12(7):914-26. doi: 10.1101/gad.12.7.914.
4
There's the rub: a novel ubiquitin-like modification linked to cell cycle regulation.问题就在这里：一种与细胞周期调控相关的新型类泛素修饰。
Genes Dev. 1998 Apr 1;12(7):901-7. doi: 10.1101/gad.12.7.901.
5
Cdc53 is a scaffold protein for multiple Cdc34/Skp1/F-box proteincomplexes that regulate cell division and methionine biosynthesis in yeast.Cdc53是多种Cdc34/Skp1/F-box蛋白复合物的支架蛋白，这些复合物在酵母中调节细胞分裂和甲硫氨酸生物合成。
Genes Dev. 1998 Mar 1;12(5):692-705. doi: 10.1101/gad.12.5.692.
6
The ubiquitin-like proteins SMT3 and SUMO-1 are conjugated by the UBC9 E2 enzyme.类泛素蛋白SMT3和SUMO-1由E2酶UBC9缀合。
Proc Natl Acad Sci U S A. 1998 Jan 20;95(2):560-4. doi: 10.1073/pnas.95.2.560.
7
Conjugation with the ubiquitin-related modifier SUMO-1 regulates the partitioning of PML within the nucleus.与泛素相关修饰因子SUMO-1的缀合作用调节了早幼粒细胞白血病蛋白（PML）在细胞核内的分布。
EMBO J. 1998 Jan 2;17(1):61-70. doi: 10.1093/emboj/17.1.61.
8
Roles of ubiquitin-mediated proteolysis in cell cycle control.泛素介导的蛋白质水解在细胞周期调控中的作用。
Curr Opin Cell Biol. 1997 Dec;9(6):788-99. doi: 10.1016/s0955-0674(97)80079-8.
9
Pathways of ubiquitin conjugation.泛素缀合途径。
FASEB J. 1997 Dec;11(14):1257-68. doi: 10.1096/fasebj.11.14.9409544.
10
The ubiquitin system.泛素系统
Trends Biochem Sci. 1997 Oct;22(10):383-7. doi: 10.1016/s0968-0004(97)01122-5.
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