Mai B, Frey G, Swanson R V, Mathur E J, Stetter K O
Lehrstuhl für Mikrobiologie, Universität Regensburg, 93053 Regensburg, Germany.
J Bacteriol. 1998 Aug;180(16):4030-5. doi: 10.1128/JB.180.16.4030-4035.1998.
An open reading frame coding for a putative protein-serine/threonine phosphatase was identified in the hyperthermophilic archaeon Pyrodictium abyssi TAG11 and named Py-PP1. Py-PP1 was expressed in Escherichia coli, purified from inclusion bodies, and biochemically characterized. The phosphatase gene is part of an operon which may provide, for the first time, insight into a physiological role for archaeal protein phosphatases in vivo.
在嗜热古菌深渊热网菌TAG11中鉴定出一个编码假定蛋白丝氨酸/苏氨酸磷酸酶的开放阅读框,并将其命名为Py-PP1。Py-PP1在大肠杆菌中表达,从包涵体中纯化,并进行了生化特性分析。该磷酸酶基因是一个操纵子的一部分,这可能首次为古菌蛋白磷酸酶在体内的生理作用提供见解。