Koelle D M, Frank J M, Johnson M L, Kwok W W
Department of Medicine, University of Washington, Seattle, Washington 98105, USA.
J Virol. 1998 Sep;72(9):7476-83. doi: 10.1128/JVI.72.9.7476-7483.1998.
The local cellular immune response to herpes simplex virus (HSV) is important in the control of recurrent HSV infection. The antiviral functions of infiltrating CD4-bearing T cells may include cytotoxicity, inhibition of viral growth, lymphokine secretion, and support of humoral and CD8 responses. The antigens recognized by many HSV-specific CD4 T cells localizing to genital HSV-2 lesions are unknown. T cells recognizing antigens encoded within map units 0. 67 to 0.73 of HSV DNA are frequently recovered from herpetic lesions. Expression cloning with this region of DNA now shows that tegument protein VP22 and the viral dUTPase, encoded by genes UL49 and UL50, respectively, are T-cell antigens. Separate epitopes in VP22 were defined for T-cell clones from each of three patients. Reactivity with the tegument protein encoded by UL21 was identified for an additional patient. Three new epitopes were identified in VP16, a tegument protein associated with VP22. Some tegument-specific CD4 T-cell clones exhibited cytotoxic activity against HSV-infected cells. These results suggest that herpes simplex tegument proteins are processed for antigen presentation in vivo and are possible candidate compounds for herpes simplex vaccines.
对单纯疱疹病毒（HSV）的局部细胞免疫反应在复发性HSV感染的控制中很重要。浸润的携带CD4的T细胞的抗病毒功能可能包括细胞毒性、抑制病毒生长、分泌淋巴因子以及支持体液免疫和CD8反应。许多定位于生殖器HSV - 2病变部位的HSV特异性CD4 T细胞所识别的抗原尚不清楚。从疱疹性病变中经常可回收识别HSV DNA图谱单位0.67至0.73内编码抗原的T细胞。利用该DNA区域进行的表达克隆现在表明，分别由基因UL49和UL50编码的被膜蛋白VP22和病毒dUTPase是T细胞抗原。为三名患者各自的T细胞克隆确定了VP22中的不同表位。还确定了另一名患者对由UL21编码的被膜蛋白具有反应性。在与VP22相关的被膜蛋白VP16中鉴定出三个新表位。一些被膜特异性CD4 T细胞克隆对HSV感染的细胞表现出细胞毒性活性。这些结果表明，单纯疱疹病毒被膜蛋白在体内被加工用于抗原呈递，并且是单纯疱疹疫苗可能的候选化合物。
