Torroella-Kouri M, Morsberger S, Carrillo A, Mohar A, Meneses A, Ibarra M, Daniel R W, Ghaffari A M, Solorza G, Shah K V
Instituto Nacional de Cancerologia, San Fernando, Tlalpan, Mexico, DF.
Gynecol Oncol. 1998 Jul;70(1):115-20. doi: 10.1006/gyno.1998.5055.
Genital human papillomavirus (HPV) infection is causally linked to cervical cancer, yet little is known regarding HPV prevalence in cancerous and normal women in Mexico, a country with a high cervical cancer incidence. We studied 185 Mexican women among the patients attending gynecological outpatient clinics in four hospitals in Mexico City. Each woman had a Pap smear, a colposcopy, and, when necessary, a biopsy. HPVs were identified by a consensus-primer-based polymerase chain reaction (PCR) assay. HPV was detected in 87% of 69 cancers, 83% of 24 high-grade squamous intraepithelial lesions (HGSILs), 33% of 21 low-grade squamous intraepithelial lesions (LGSILs), and 17% of 71 normals. Twenty-one of the 32 HPV types tested were detected at least once. The ratio of high-risk:low-risk types was 87:6 in HGSILs and cancers, compared to 11:8 for LGSILs and normals. In invasive cancers, HPV types found at the highest frequency were, in descending order: HPV-16, -18, and -45, followed by -39, -59, and -58 with the same frequency. HPV-16 and related types were present in 52% of the cancer cases, as well as in 79% of HGSILs, and HPV-18 and related types were present in 36% of the cancers but in only 12.5% of the HGSILs. HPV-16 was predominant in squamous carcinomas, and HPV-18 and related types were predominant in adenosquamous carcinoma. Both biopsies and scrapes were tested for HPVs in 63 women, all of them with cervical neoplasia. Identical HPV results were obtained in 89% of the samples, but additional types were often identified in scrapes. HPV prevalence and type distribution in cervical cancer in Mexico was similar to the reported worldwide, as well as in other Latin American countries.
生殖器人乳头瘤病毒（HPV）感染与宫颈癌存在因果关联，但对于宫颈癌发病率较高的墨西哥，患癌女性与正常女性中HPV的流行情况却知之甚少。我们对墨西哥城四家医院妇科门诊的185名墨西哥女性患者进行了研究。每位女性均接受了巴氏涂片检查、阴道镜检查，必要时还进行了活检。通过基于共识引物的聚合酶链反应（PCR）检测法来鉴定HPV。在69例癌症患者中，87%检测出HPV；24例高级别鳞状上皮内病变（HGSIL）患者中，83%检测出HPV；21例低级别鳞状上皮内病变（LGSIL）患者中，33%检测出HPV；71例正常女性中，17%检测出HPV。所检测的32种HPV类型中，有21种至少被检测到一次。在HGSIL和癌症患者中，高危型与低危型的比例为87:6，而在LGSIL和正常女性中这一比例为11:8。在浸润性癌症中，出现频率最高的HPV类型依次为：HPV - 16、- 18和- 45，随后是频率相同的- 39、- 59和- 58。52%的癌症病例以及79%的HGSIL中存在HPV - 16及相关类型，36%的癌症病例中存在HPV - 18及相关类型，但仅12.5%的HGSIL中存在该类型。HPV - 16在鳞状细胞癌中占主导，HPV - 18及相关类型在腺鳞癌中占主导。对63例宫颈肿瘤女性患者的活检组织和刮片样本都进行了HPV检测，89%的样本获得了相同的HPV检测结果，但刮片中常常能检测出更多类型的HPV。墨西哥宫颈癌患者中HPV的流行情况和类型分布与全球及其他拉丁美洲国家所报告的情况相似。
