Honikel K O, Hambloch H
Z Lebensm Unters Forsch. 1976 Aug 25;161(4):337-46. doi: 10.1007/BF01113957.
Antibiotics in biological materials, including tetracyclines, are determined usually be means of microbiological methods. Because of the low specificity and the time consuming procedure of the microbiological assays, we developed a fluorimetric method, which enables us to determine specifically the tetracyclines and to reduce the time for the assay down to 4 hours. Our method is based on the determination of tetracyclines published by Kohn (1961), which we modified for the determination of chlortetracycline in meat and bone. Tetracyclines are fluorescent compounds, whose quantum yields are very much enhanced in most cases by forming a complex with Ca2+ ions and by extracting it into an organic solvent. This transfer into acetic acid ethylester separates the chlortetracycline from interfering substances. The yield of chlortetracycline with the described method was about 80%, the sensitivity of the detection was about 0.5 ppm.
生物材料中的抗生素，包括四环素，通常采用微生物学方法进行测定。由于微生物学检测特异性低且过程耗时，我们开发了一种荧光法，该方法能使我们特异性地测定四环素，并将检测时间缩短至4小时。我们的方法基于科恩（1961年）发表的四环素测定方法，我们对其进行了修改以用于测定肉和骨中的金霉素。四环素是荧光化合物，在大多数情况下，通过与钙离子形成复合物并将其萃取到有机溶剂中，其量子产率会大大提高。转移到乙酸乙酯中可将金霉素与干扰物质分离。用所述方法测定金霉素的回收率约为80%，检测灵敏度约为0.5 ppm。
