Tamaki H, Akamine T, Goshi N, Kurata H, Sakou T
Department of Orthopaedic Surgery, Faculty of Medicine, Kagoshima University, Kanoya, Japan.
Bone. 1998 Aug;23(2):147-53. doi: 10.1016/s8756-3282(98)00075-1.
This study was designed to assess the effects of exercise training (Tr) following an etidronate treatment (E) on bone mineral density (BMD) of the femur and trabecular bone of the tibia in ovariectomized (ovx) rats. Female Wistar rats were ovariectomized (ovx) or sham-operated (sham) at 15 weeks of age and divided into five experimental groups: sham; ovx; ovx + E; ovx + Tr; ovx + E + Tr. Etidronate treatment of 5 mg/kg, 5 days/week was administered for 2 weeks and exercised on a treadmill for 30 m/min, 60 min/day, 5 days/week for 10 weeks. BMD of the femur and the trabecular bone area of the proximal tibia were significantly (p < 0.05) higher in E and/or Tr compared to ovx groups. However, the cortical region was not affected significantly by ovariectomy. The area partially filled with the trabecular bone at the constant width was observed only in the E rats. The number of osteoclasts in E group was significantly lower (p < 0.05) than in the ovx and ovx + Tr groups. The ovx + Tr rats had a higher number of osteoblasts (p < 0.05) than the ovx and ovx + E groups. There was a significant interaction between ovx + Tr and ovx + E on BMD in the proximal region of the femur (p < 0.05) and trabecular bone area of the tibia (p < 0.001). These results suggest that the etidronate treatment for 2 weeks beforehand influenced the effects of subsequent exercise training on maintaining the BMD in the proximal femur and the trabecular bone area of the tibia.
本研究旨在评估依替膦酸治疗（E）后进行运动训练（Tr）对去卵巢（ovx）大鼠股骨骨密度（BMD）及胫骨小梁骨的影响。15周龄雌性Wistar大鼠行去卵巢（ovx）或假手术（sham），分为五个实验组：假手术组；去卵巢组；去卵巢 + E组；去卵巢 + Tr组；去卵巢 + E + Tr组。依替膦酸按5 mg/kg、每周5天给药，持续2周，在跑步机上以30 m/min的速度运动，每天60 min、每周5天，持续10周。与去卵巢组相比，E组和/或Tr组的股骨BMD及胫骨近端小梁骨面积显著更高（p < 0.05）。然而，皮质区域未受去卵巢的显著影响。仅在E组大鼠中观察到在恒定宽度处部分填充有小梁骨的区域。E组破骨细胞数量显著低于去卵巢组和去卵巢 + Tr组（p < 0.05）。去卵巢 + Tr组的成骨细胞数量高于去卵巢组和去卵巢 + E组（p < 0.05）。在股骨近端区域的BMD（p < 0.05）及胫骨小梁骨面积（p < 0.001）方面，去卵巢 + Tr组与去卵巢 + E组之间存在显著交互作用。这些结果表明，预先进行2周的依替膦酸治疗会影响后续运动训练对维持股骨近端和胫骨小梁骨区域BMD的效果。
