γ-氨基丁酸从神经元和神经胶质细胞的释放。

Gamma-aminobutyric acid release from neurons and glia.

作者信息

Sellström A, Hamberger A

出版信息

Acta Physiol Scand. 1976 Sep;98(1):94-102. doi: 10.1111/j.1748-1716.1976.tb10307.x.

DOI:10.1111/j.1748-1716.1976.tb10307.x

Abstract

Release of 3H-GABA into the medium from bulk-isolated glial cells, neuronal perikarya and synaptosomes has been studied, using a continuous perfusion system. The fractions were incubated with 3H-GABA and spontaneous efflux of label in the presence and absence of aminooxyacetic acid (AOAA) was recorded. Unlabelled GABA at micromolar concentrations markedly stimulated 3H-GABA efflux from glia, synaptosomes and neurons. 3H-GABA from all fractions was also markedly sensitive to stimulation by ouabain. Glutamate was effective in stimulating the spontaneous efflux in the presence of AOAA, while superfusion with a calcium-deprived medium only slightly stimulated the 3H-GABA efflux from neurons and glia.

摘要

利用连续灌注系统，对从大量分离的神经胶质细胞、神经元胞体和突触体中释放到培养基中的3H-γ-氨基丁酸（GABA）进行了研究。将这些组分与3H-GABA一起孵育，并记录在有和没有氨氧基乙酸（AOAA）存在的情况下标记物的自发流出。微摩尔浓度的未标记GABA显著刺激了神经胶质细胞、突触体和神经元中3H-GABA的流出。来自所有组分的3H-GABA对哇巴因的刺激也非常敏感。在AOAA存在的情况下，谷氨酸能有效刺激自发流出，而用无钙培养基进行灌流仅轻微刺激神经元和神经胶质细胞中3H-GABA的流出。