Riadnov M G, Kashparova N Ia, Kashparov I A, Mitin Iu V
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow oblast, Russia.
Bioorg Khim. 1998 Jun;24(6):408-11.
In order to increase the solubility of free amino acids so that they could be used in peptide synthesis, a system consisting of dimethylformamide, an inorganic additive, and pyridine was suggested. Compounds BF3, AlCl3, SnCl2, SiCl4, ZnCl2, SbCl3, CaCl2, BaI2, CdI2, and NaI can serve as the additives. Acylation of amino acids in such a solution (pH 5.2-5.3) with pentafluorophenyl esters of N alpha-protected amino acids gave N alpha-protected di- and tripeptides in a yield of more than 90%.
为了提高游离氨基酸的溶解度以便用于肽合成，有人提出了一种由二甲基甲酰胺、一种无机添加剂和吡啶组成的体系。化合物BF3、AlCl3、SnCl2、SiCl4、ZnCl2、SbCl3、CaCl2、BaI2、CdI2和NaI可作为添加剂。在这样的溶液（pH 5.2 - 5.3）中，用Nα-保护氨基酸的五氟苯基酯对氨基酸进行酰化反应，得到产率超过90%的Nα-保护二肽和三肽。
