一种新型的β-血影蛋白脑特异性同工型：分离及其与Munc13的相互作用。

A novel brain-specific isoform of beta spectrin: isolation and its interaction with Munc13.

作者信息

Sakaguchi G, Orita S, Naito A, Maeda M, Igarashi H, Sasaki T, Takai Y

机构信息

Shionogi Institute for Medical Science, Settsu, Japan.

出版信息

Biochem Biophys Res Commun. 1998 Jul 30;248(3):846-51. doi: 10.1006/bbrc.1998.9067.

DOI:10.1006/bbrc.1998.9067

PMID:9704016

Abstract

Munc13 is a component of the neurotransmitter release machinery which is specifically expressed in brain. Munc13 interacts with Doc2 and syntaxin which are also implicated in the neurotransmitter release process. Here we isolated another Munc13-interacting molecule from a rat brain cDNA library by use of the yeast two-hybrid system, identified it to be a novel type of beta spectrin, and named it beta SpIII sigma 1. beta SpIII sigma 1 was specifically expressed in brain, where it was enriched in the synaptic vesicle and plasma membrane fractions. Because spectrin has been shown to interact with the actin cytoskeleton which is involved in the exocytotic process, the present results suggest that the Munc13-beta SpIII sigma 1 interactions play a role in neurotransmitter release.

摘要

Munc13是神经递质释放机制的一个组成部分，在大脑中特异性表达。Munc13与同样参与神经递质释放过程的Doc2和 syntaxin相互作用。在这里，我们利用酵母双杂交系统从大鼠脑cDNA文库中分离出另一个与Munc13相互作用的分子，鉴定其为一种新型的β-血影蛋白，并将其命名为βSpIIIσ1。βSpIIIσ1在大脑中特异性表达，在突触小泡和质膜组分中富集。由于血影蛋白已被证明与参与胞吐过程的肌动蛋白细胞骨架相互作用，目前的结果表明Munc13-βSpIIIσ1相互作用在神经递质释放中起作用。