• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis.

作者信息

Chow G, Nietfeld J J, Knudson C B, Knudson W

机构信息

Rush Medical College, Rush-Presbyterian-St. Luke's Medical Center, Chicago, Illinois 60612, USA.

出版信息

Arthritis Rheum. 1998 Aug;41(8):1411-9. doi: 10.1002/1529-0131(199808)41:8<1411::AID-ART10>3.0.CO;2-Z.

DOI:10.1002/1529-0131(199808)41:8<1411::AID-ART10>3.0.CO;2-Z
PMID:9704639
Abstract

OBJECTIVE

To better define critical functions of the hyaluronan receptor CD44 in cartilage.

METHODS

Articular chondrocytes and cartilage tissue slices were treated with CD44 sequence-specific antisense phosphorothioate oligonucleotides. CD44 expression was probed by immunofluorescence microscopy, enzyme-linked immunosorbent assay, and Western blotting.

RESULTS

Antisense oligonucleotides demonstrated a dose- and time-dependent inhibition of CD44 protein expression; negative controls showed no effect. Similar to osteoarthritic cartilage, antisense-treated cartilage slices displayed a near-total loss of stainable proteoglycan-rich matrix.

CONCLUSION

CD44 expression is needed for maintenance of cartilage homeostasis.

摘要

相似文献

1
Antisense inhibition of chondrocyte CD44 expression leading to cartilage chondrolysis.
Arthritis Rheum. 1998 Aug;41(8):1411-9. doi: 10.1002/1529-0131(199808)41:8<1411::AID-ART10>3.0.CO;2-Z.
2
Latrunculin and cytochalasin decrease chondrocyte matrix retention.拉特罗毒素和细胞松弛素会降低软骨细胞的基质保留能力。
J Histochem Cytochem. 2002 Oct;50(10):1313-24. doi: 10.1177/002215540205001004.
3
Antisense oligonucleotides to the integrin receptor subunit alpha(5) decrease fibronectin fragment mediated cartilage chondrolysis.针对整合素受体亚基α(5)的反义寡核苷酸可减少纤连蛋白片段介导的软骨溶解。
Osteoarthritis Cartilage. 2002 May;10(5):381-93. doi: 10.1053/joca.2002.0524.
4
Antisense inhibition of CD44 tailless splice variant in human articular chondrocytes promotes hyaluronan internalization.对人类关节软骨细胞中CD44无尾剪接变体的反义抑制可促进透明质酸内化。
Arthritis Rheum. 2001 Nov;44(11):2599-610. doi: 10.1002/1529-0131(200111)44:11<2599::aid-art440>3.0.co;2-y.
5
The accumulation of intracellular ITEGE and DIPEN neoepitopes in bovine articular chondrocytes is mediated by CD44 internalization of hyaluronan.牛关节软骨细胞内ITEGE和DIPEN新表位的积累是由透明质酸的CD44内化介导的。
Arthritis Rheum. 2006 Feb;54(2):443-54. doi: 10.1002/art.21623.
6
Hyaluronan oligosaccharides perturb cartilage matrix homeostasis and induce chondrocytic chondrolysis.透明质酸寡糖扰乱软骨基质稳态并诱导软骨细胞溶解。
Arthritis Rheum. 2000 May;43(5):1165-74. doi: 10.1002/1529-0131(200005)43:5<1165::AID-ANR27>3.0.CO;2-H.
7
Stimulation of hyaluronan metabolism by interleukin-1alpha in human articular cartilage.白细胞介素-1α对人关节软骨透明质酸代谢的刺激作用
Arthritis Rheum. 2000 Jun;43(6):1315-26. doi: 10.1002/1529-0131(200006)43:6<1315::AID-ANR14>3.0.CO;2-#.
8
CD44 knock-down in bovine and human chondrocytes results in release of bound HYAL2.在牛和人软骨细胞中敲低CD44会导致结合的HYAL2释放。
Matrix Biol. 2015 Oct;48:42-54. doi: 10.1016/j.matbio.2015.04.002. Epub 2015 Apr 9.
9
Osteogenic protein-1 promotes the synthesis and retention of extracellular matrix within bovine articular cartilage and chondrocyte cultures.成骨蛋白-1促进牛关节软骨和软骨细胞培养物中细胞外基质的合成与保留。
Osteoarthritis Cartilage. 2000 Mar;8(2):127-36. doi: 10.1053/joca.1999.0281.
10
Inhibition of interleukin-1beta-stimulated production of matrix metalloproteinases by hyaluronan via CD44 in human articular cartilage.透明质酸通过CD44抑制白细胞介素-1β刺激人关节软骨中基质金属蛋白酶的产生。
Arthritis Rheum. 2004 Feb;50(2):516-25. doi: 10.1002/art.20004.

引用本文的文献

1
Development of the mechanoresponsive pericellular matrix of chondrons.软骨细胞周围机械响应性基质的发育
Sci Adv. 2025 May 2;11(18):eado6644. doi: 10.1126/sciadv.ado6644.
2
Comparison between articular chondrocytes and mesenchymal stromal cells for the production of articular cartilage implants.用于生产关节软骨植入物的关节软骨细胞与间充质基质细胞的比较。
Front Bioeng Biotechnol. 2023 Feb 21;11:1116513. doi: 10.3389/fbioe.2023.1116513. eCollection 2023.
3
Engineering Hyaluronic Acid for the Development of New Treatment Strategies for Osteoarthritis.
工程化透明质酸用于开发骨关节炎新的治疗策略。
Int J Mol Sci. 2022 Aug 4;23(15):8662. doi: 10.3390/ijms23158662.
4
Single-cell rna seq analysis identifies the biomarkers and differentiation of chondrocyte in human osteoarthritis.单细胞RNA测序分析确定人类骨关节炎中软骨细胞的生物标志物和分化情况。
Am J Transl Res. 2020 Nov 15;12(11):7326-7339. eCollection 2020.
5
Synovial membrane mesenchymal stem cells: past life, current situation, and application in bone and joint diseases.滑膜间充质干细胞：前世今生，及其在骨关节疾病中的应用。
Stem Cell Res Ther. 2020 Sep 7;11(1):381. doi: 10.1186/s13287-020-01885-3.
6
Hydrogel Containing Anti-CD44-Labeled Microparticles, Guide Bone Tissue Formation in Osteochondral Defects in Rabbits.含抗CD44标记微粒的水凝胶引导兔骨软骨缺损处骨组织形成。
Nanomaterials (Basel). 2020 Jul 31;10(8):1504. doi: 10.3390/nano10081504.
7
Anti-Inflammatory Effects of Intra-Articular Hyaluronic Acid: A Systematic Review.关节腔内透明质酸的抗炎作用：一项系统评价
Cartilage. 2019 Jan;10(1):43-52. doi: 10.1177/1947603517749919. Epub 2018 Feb 11.
8
P38 mitogen-activated protein kinase promotes dedifferentiation of primary articular chondrocytes in monolayer culture.P38 丝裂原活化蛋白激酶促进单层培养中原代关节软骨细胞的去分化。
J Cell Mol Med. 2013 Apr;17(4):508-17. doi: 10.1111/jcmm.12034. Epub 2013 Mar 11.
9
Culture of primary bovine chondrocytes on a continuously expanding surface inhibits dedifferentiation.在不断扩大的表面上培养原代牛软骨细胞可抑制去分化。
Tissue Eng Part A. 2012 Dec;18(23-24):2466-76. doi: 10.1089/ten.TEA.2012.0215. Epub 2012 Aug 3.
10
Induction of CD44 cleavage in articular chondrocytes.诱导关节软骨细胞中CD44的裂解
Arthritis Rheum. 2010 May;62(5):1338-48. doi: 10.1002/art.27410.