Kawagoe T, Nakao A, Kanbe T, Tamura H, Tanaka S, Takagi H
Department of Surgery II, Nagoya University School of Medicine, Japan.
Eur Surg Res. 1998;30(4):290-6. doi: 10.1159/000008589.
To investigate the utility of measuring blood concentrations of (1-->3)-beta-D-glucan, a component of the fungal cell wall, as an auxiliary diagnostic method for systemic candidiasis, rats were inoculated with Candida albicans and the number of C. albicans in the viscera and glucan in the blood were quantitated. The concentration of blood glucan and the number of C. albicans in the viscera were also measured both under leukopenia and with deteriorated reticuloendothelial system cell function, and when the liver and spleen had been excised. As a result, systemic candidiasis appeared in the group with leukopenia, and the number of living C. albicans increased in the kidney and liver. Together with this increase in the number of C. albicans, there was an increase in blood (1--> 3)-beta-D-glucan. Measurements of blood (1--> 3)-beta-D-glucan well reflect a proliferation of C. albicans in vivo, which would make this a useful auxiliary for the clinical diagnosis of systemic mycosis.
为了研究测量真菌细胞壁成分（1→3）-β-D-葡聚糖的血药浓度作为系统性念珠菌病辅助诊断方法的效用，给大鼠接种白色念珠菌，并对内脏中白色念珠菌的数量和血液中葡聚糖的含量进行定量。在白细胞减少、网状内皮系统细胞功能恶化以及肝脾切除的情况下，也测量了血液葡聚糖浓度和内脏中白色念珠菌的数量。结果，白细胞减少组出现了系统性念珠菌病，肾脏和肝脏中活的白色念珠菌数量增加。随着白色念珠菌数量的增加，血液中（1→3）-β-D-葡聚糖也增加。血液中（1→3）-β-D-葡聚糖的测量能很好地反映体内白色念珠菌的增殖情况，这使其成为系统性真菌病临床诊断的有用辅助手段。
