通过快速离心培养和传统试管细胞培养检测临床标本中的腮腺炎病毒。

Detection of mumps virus in clinical specimens by rapid centrifugation culture and conventional tube cell culture.

作者信息

Germann D, Gorgievski M, Ströhle A, Matter L

机构信息

Institute of Medical Microbiology, Division of Clinical Microbiology, University of Bern, Switzerland.

出版信息

J Virol Methods. 1998 Jul;73(1):59-64. doi: 10.1016/s0166-0934(98)00038-x.

DOI:10.1016/s0166-0934(98)00038-x

PMID:9705175

Abstract

Conventional tube cell culture was compared with a 2 day and 5 day spin-amplified shell vial indirect immunofluorescence assay for the detection of mumps virus in swabs from the area of Stensen's duct. The sensitivity and specificity of the shell vial assay were 95.9 and 100%, respectively. The shell vial detected 66.3% of the positive cultures within 2 days of inoculation while the first positive results were available by conventional tube cell culture after 3 days (1.6%) reaching 72.4% of all culture positive specimens after 7 days. These data suggest that a centrifugation shell vial indirect immunofluorescence assay may be useful for rapid detection of mumps virus in clinical specimens.

摘要

将传统的试管细胞培养法与为期2天和5天的旋转扩增空斑小瓶间接免疫荧光测定法进行比较，以检测来自腮腺导管区域拭子中的腮腺炎病毒。空斑小瓶测定法的灵敏度和特异性分别为95.9%和100%。空斑小瓶在接种后2天内检测出66.3%的阳性培养物，而传统试管细胞培养在3天后才得到首个阳性结果（1.6%），7天后达到所有培养阳性标本的72.4%。这些数据表明，离心空斑小瓶间接免疫荧光测定法可能有助于临床标本中腮腺炎病毒的快速检测。