Agha S A, Coleman J C, Mahmoud L A, Abd-Elaal A M, Selwyn S
Department of Medical Microbiology, Charing Cross and Westminster Medical School, London, England.
J Med Virol. 1988 Sep;26(1):85-92. doi: 10.1002/jmv.1890260112.
Conventional tube cell culture has been recognised as the most sensitive technique available for human cytomegalovirus (HCMV) detection. Low-speed centrifugation of specimen inocula onto cell culture monolayers has been shown to increase the efficiency of infection with the AD 169 strain of HCMV. Therefore a centrifugal force of 900g for 1 hour at 37 degrees C was used to enhance the detection of HCMV cytopathic effect (CPE) in shell vials that contained circular coverslips with a monolayer of human embryonic lung (HEL) fibroblasts. Of 195 specimens, HCMV CPE was detected in 18 specimens (9.02%) on shell vial culture assay, whereas conventional tube cell culture was positive in only 13 specimens (6.6%). The shell vial culture assay was significantly more sensitive (P less than 0.05). Furthermore the development of the cytopathic effect on shell vial culture assay was significantly earlier (P less than 0.01) and more extensive. Urine samples were sonicated and the results obtained with immunofluorescence using human immune serum demonstrated that sonication increased both the intensity of fluorescence and number of fluorescent foci of HCMV-infected cells and also decreased the non-specific fluorescence of the background.
传统的试管细胞培养已被公认为是检测人巨细胞病毒（HCMV）最敏感的技术。已证明将标本接种物低速离心到细胞培养单层上可提高HCMV AD 169株的感染效率。因此，在37℃下以900g的离心力离心1小时，以增强在含有单层人胚肺（HEL）成纤维细胞圆形盖玻片的壳瓶中对HCMV细胞病变效应（CPE）的检测。在195份标本中，壳瓶培养检测在18份标本（9.02%）中检测到HCMV CPE，而传统的试管细胞培养仅在13份标本（6.6%）中呈阳性。壳瓶培养检测的敏感性显著更高（P<0.05）。此外，壳瓶培养检测中细胞病变效应的出现明显更早（P<0.01）且更广泛。对尿液样本进行超声处理，使用人免疫血清通过免疫荧光获得的结果表明，超声处理增加了HCMV感染细胞的荧光强度和荧光灶数量，同时也降低了背景的非特异性荧光。
