无洗涤剂培养基中分枝杆菌生长快速测量的新方法。
Novel method for rapid measurement of growth of mycobacteria in detergent-free media.
作者信息
Meyers P R, Bourn W R, Steyn L M, van Helden P D, Beyers A D, Brown G D
机构信息
Department of Medical Microbiology, University of Cape Town, Observatory, Cape Town 7925, South Africa.
出版信息
J Clin Microbiol. 1998 Sep;36(9):2752-4. doi: 10.1128/JCM.36.9.2752-2754.1998.
Abstract
We describe a novel, rapid, and inexpensive method for the measurement of growth of Mycobacterium tuberculosis, Mycobacterium bovis, and Mycobacterium smegmatis in the presence or absence of detergent. The method, which employs hot NaOH treatment of mycobacterial cells to release total cellular protein, compares favorably with other methods for monitoring mycobacterial growth but is particularly useful for heavily clumped cultures grown in defined minimal medium.
摘要
我们描述了一种新颖、快速且廉价的方法，用于在有或没有去污剂存在的情况下测量结核分枝杆菌、牛分枝杆菌和耻垢分枝杆菌的生长情况。该方法采用热氢氧化钠处理分枝杆菌细胞以释放总细胞蛋白，与其他监测分枝杆菌生长的方法相比具有优势，尤其适用于在限定的基本培养基中生长的严重结块培养物。
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