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用于区分乳房链球菌和副乳房链球菌的种特异性寡核苷酸探针的开发与应用。

Development and use of species-specific oligonucleotide probes for differentiation of Streptococcus uberis and Streptococcus parauberis.

作者信息

Bentley R W, Leigh J A, Collins M D

机构信息

AFRC Institute for Animal Health, Compton, Newbury, United Kingdom.

出版信息

J Clin Microbiol. 1993 Jan;31(1):57-60. doi: 10.1128/jcm.31.1.57-60.1993.

DOI:10.1128/jcm.31.1.57-60.1993
PMID:8417033
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC262621/
Abstract

Oligonucleotide probes specific for 16S rRNA and capable of differentiating Streptococcus uberis and S. parauberis from each other and other esculin-hydrolyzing streptococci were developed. Use of a mini-RNA extraction technique for gram-positive cocci associated with bovine mastitis has allowed the probes to be used for identification of esculin-hydrolyzing streptococci from two dairy herds at the Institute for Animal Health, Compton, United Kingdom. One hundred seventy-nine of 206 isolates were identified as S. uberis, 3 were identified as S. parauberis, and 24 were not identified. Isolates not identified by the probes were tested biochemically and found to be mainly Enterococcus faecium, E. faecalis, or S. bovis.

摘要

开发了针对16S rRNA的寡核苷酸探针，该探针能够区分乳房链球菌和副乳房链球菌以及其他水解七叶苷的链球菌。使用一种针对与牛乳腺炎相关的革兰氏阳性球菌的微型RNA提取技术，使得这些探针可用于鉴定英国康普顿动物健康研究所两个奶牛场中水解七叶苷的链球菌。206株分离株中有179株被鉴定为乳房链球菌，3株被鉴定为副乳房链球菌，24株未被鉴定。未被探针鉴定的分离株经生化测试，发现主要是粪肠球菌、屎肠球菌或牛链球菌。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c7/262621/0bc5dc3def95/jcm00013-0077-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c7/262621/052ea223fec7/jcm00013-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c7/262621/0bc5dc3def95/jcm00013-0077-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c7/262621/052ea223fec7/jcm00013-0077-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/f1c7/262621/0bc5dc3def95/jcm00013-0077-b.jpg

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1
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Proc Natl Acad Sci U S A. 1983 Jan;80(1):278-82. doi: 10.1073/pnas.80.1.278.
2
Evaluation of the Rapid Strep system for the identification of clinical isolates of Streptococcus species.用于鉴定链球菌属临床分离株的快速链球菌检测系统的评估。
J Clin Microbiol. 1984 Nov;20(5):894-8. doi: 10.1128/jcm.20.5.894-898.1984.
3
On the evolutionary descent of organisms and organelles: a global phylogeny based on a highly conserved structural core in small subunit ribosomal RNA.
链球菌怎么了：分类学和命名法变化概述
Clin Microbiol Rev. 2002 Oct;15(4):613-30. doi: 10.1128/CMR.15.4.613-630.2002.
4
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5
Development of specific nested oligonucleotide PCR primers for the Streptococcus iniae 16S-23S ribosomal DNA intergenic spacer.海豚链球菌16S - 23S核糖体DNA基因间隔区特异性巢式寡核苷酸PCR引物的研制。
J Clin Microbiol. 1998 Sep;36(9):2778-81. doi: 10.1128/JCM.36.9.2778-2781.1998.
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4
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5
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6
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7
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8
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9
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10
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