The effect of dissociative anaesthesia with ketamine on the activity of lysosomal enzymes in peripheral blood granulocytes of guinea pigs.

The effect of ketamine on the activity of lysosomal enzymes in peripheral blood granulocytes was studied in guinea pigs. Ketamine was given intramuscularly in one single dose, in divided doses and in long-term administration (repeatedly) for 14 days. The blood for determination was obtained from the heart, the lysosomes were prepared by the Chodirker's method determining the activity of marker enzymes: acid phosphatase, acid protease and neutral protease, in the obtained fractions. No statistically significant differences were found in the activity of lysosomal enzymes in the studied groups. The results obtained point out the usefulness of ketamine in severe conditions associated with significant catabolic changes in the organism.