Tynkkynen S, Singh K V, Varmanen P
Valio Ltd, R&D, Finland.
Int J Food Microbiol. 1998 Jun 16;41(3):195-204. doi: 10.1016/s0168-1605(98)00051-8.
Lactobacillus rhamnosus GG (ATCC 53103) is a probiotic strain used in fermented dairy products in many countries and is also used as a food supplement in the form of freeze-dried powder. The relationship of the vancomycin resistance factor in L. rhamnosus GG and the vancomycin resistance (van) genes of Enterococcus faecalis and E. faecium were studied using polymerase chain reaction (PCR), Southern hybridization and conjugation methods. Our results show that the vancomycin resistance determinant in L. rhamnosus GG is not closely related to enterococcal van genes, since no PCR product was amplified in L. rhamnosus GG with any of the three sets of vanA primers used, and enterococcal vanA, vanB, vnH, vanX, vanZ, vanY, vanS and vanR genes did not hybridize with DNA of L. rhamnosus GG. This strain does not contain plasmids and transfer of chromosomal vancomycin resistance determinant from L. rhamnosus GG to enterococcal species was not detected. Our results are in accordance with previous findings of intrinsically vancomycin-resistant lactic acid bacteria.
鼠李糖乳杆菌GG（ATCC 53103）是一种益生菌菌株，在许多国家被用于发酵乳制品，也以冻干粉末的形式用作食品补充剂。使用聚合酶链反应（PCR）、Southern杂交和接合方法研究了鼠李糖乳杆菌GG中的万古霉素抗性因子与粪肠球菌和屎肠球菌的万古霉素抗性（van）基因之间的关系。我们的结果表明，鼠李糖乳杆菌GG中的万古霉素抗性决定簇与肠球菌van基因没有密切关系，因为使用的三组vanA引物中的任何一组在鼠李糖乳杆菌GG中均未扩增出PCR产物，并且肠球菌的vanA、vanB、vnH、vanX、vanZ、vanY、vanS和vanR基因与鼠李糖乳杆菌GG的DNA没有杂交。该菌株不含质粒，未检测到鼠李糖乳杆菌GG的染色体万古霉素抗性决定簇向肠球菌属的转移。我们的结果与先前关于固有耐万古霉素乳酸菌的研究结果一致。
